APES处理的载玻片在原位杂交技术中的应用  

Glass Slides Treated by Improved APES Method were Used in in situ Hybridization

作　　者：滕鸿琦[1] 施珏平[1] 肖爱平[1] 李小兵[1] 张明凤[1] 

机构地区：[1]福建省发育与神经生物学重点实验室福建师范大学生命科学学院,福州350108

出　　处：《福建畜牧兽医》2011年第5期1-4,共4页Fujian Journal of Animal Husbandry and Veterinary medicine

基　　金：国家自然科学基金青年基金项目(31000643);国家自然科学基金项目(30871422);卫生部科学研究基金-福建省卫生教育联合攻关计划资助项目(WKJ2008-2-51)资助

摘　　要：目的:获得一种廉价的、核酸酶(RNase)处理效果好的载玻片运用于原位杂交技术中。方法:改进传统APES(3-Aminopropyl triethoxysilane)方法处理载玻片,加入载玻片高温烘烤、丙酮稀释APES等步骤,以去除RNase污染,并将处理好的载玻片分别进行冰冻和石蜡组织贴片,用原位杂交试验进行验证。结果和结论:原位杂交结果显示两种组织贴片效果良好,组织未出现掉片,RNA信号特异,与进口载玻片没有差异。因此,改进APES方法处理的载玻片完全能够运用于原位杂交技术中,可大大节约试验成本。Objective：To obtain a kind of cheap glass slides without RNase pollution,then could be used to in situ hybridization.Method：An improved APES（3-Aminopropyl triethoxysilane） treatment method was used to remove RNase pollution through baking glass slides at high temperature and diluting APES with acetone.These glass slides were used to stick the paraffin and frozen tissues,then the experimental effects of these glass slides were evaluated by in situ hybridization.Results and Conclusion：Both paraffin and frozen sections were well fit for in situ hybridization without any ecclasis of tissues,and specific RNA signals were detected.Our findings indicated that the effects of these APES-treated glass slides could be equal to the imported ones.Therefore,we concluded these APES-treated glass slides can absolutely be used to in situ hybridization,moreover,the experimental cost will be saved remarkably.

关 键 词：载玻片 APES 石蜡切片 冰冻切片 原位杂交 

分 类 号：S814.8[农业科学—畜牧学]