海兰鸡PRL、OVR、OIH和GnRHR基因多态性及其与产蛋性能的关联分析  

Association Analysis of PRL,OVR,OIH and GnRHR Gene's Polymorphisms with Laying Performances in Hy-line Chicken

作　　者：朱凯[1,2] 陈强[1,2] 张向喆[1,2] 王起山[1,2] 涂盈盈 潘玉春[1,2] 杨长锁 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海市兽医生物技术重点实验室,上海200240 [3]国家家禽工程技术研究中心,上海200331

出　　处：《中国家禽》2011年第19期31-35,共5页China Poultry

基　　金：国家高技术研究发展计划(863计划)项目(2008AA101009)

摘　　要：本研究以海兰褐与海兰白两个蛋鸡品系作为研究素材,选择催乳素基因(PRL)、卵细胞卵黄受体基因(OVR)、卵抑制剂基因(OIH)以及促性腺激素释放激素受体基因(GnRHR)4个基因作为产蛋性状相关候选基因,采用PCR-RFLP方法对候选基因进行多样性检测,并与蛋重及产蛋数两性状进行关联分析。结果表明,PRL、OVR和OIH3个基因候选位点在本研究海兰蛋鸡群体中存在SNPs,但3个基因候选位点不同基因型与产蛋性状关联分析均未能达到显著水平(P>0.05);GnRHR基因候选位点在本研究群体中未检测到多态性的存在。In this study,Hy-line brown and white laying hens were used as research materials.Prolactin gene（PRL）,occytevitellogenesis receptor gene（OVR）,ovoinhibitor gene（OIH）and gonadotropin-releasing hormone receptor gene（GnRHR） were employed as the candidate genes associated with laying traits.Diversity of candidate genes were detected using PCR-RFLP,then the detecting results were analyzed with egg weight and egg number.The results showed that SNPs existed at candidate site of PRL,OVR and OIH of Hy-line laying hen,but the effect of genotypes on laying traits were not significant for each gene.Candidate site of GnRHR did not find SNPs in this study.

关 键 词：海兰蛋鸡 候选基因 SNPS 产蛋性状 关联分析 

分 类 号：S831.5[农业科学—畜牧学]