基于16S rRNA基因序列分析受砷和硫酸盐污染的土壤细菌多样性(英文)  

Characterization of bacteria community associated with soil arsenic and sulphate contamination based on 16S rRNA gene sequences

作　　者：蒋德明[1] 孙玉华 李丹[1] 郭大雷 吴自荣[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]昆山华科生物高分子材料研究所,江苏昆山215300

出　　处：《微生物学通报》2011年第10期1592-1601,共10页Microbiology China

基　　金：supported by New Teacher Fund of East China Normal University and Daxia Cup Fund of East China Normal University

摘　　要：为了了解普通耕地土壤(Nor-1)和受砷及硫酸盐污染土壤(Sul-1)中的细菌组成和多样性差异,对2个不同土壤样品直接提取总DNA,通过PCR扩增16S rRNA基因并建立文库,对文库克隆进行核糖体DNA扩增片段酶切分析(ARDRA)和测序,构建系统进化树。从Nor-1土壤样品中测序获得23个16S rRNA基因序列,分析序列系统发育关系表明,共包含Acidobacteria(12.3%,8/65)、Actinobacteria(3.1%,2/65)、Firmicutes(21.5%,14/65)、Nitrospira(3.1%,2/65)和Proteobacteria(60%,39/65)等5个不同细菌门。而从Sul-1土壤样品中测序获得19个16S rRNA基因序列,分析序列系统发育关系表明,共包含Firmicutes(29.5%,13/44)和Proteobacteria(70.5%,31/44)等2个不同细菌门。结果表明,受高浓度的砷和硫酸盐的影响,Sul-1土壤中细菌群落结构相较于普通耕地土壤(Nor-1)发生了明显的改变,多样性明显下降,但有大量具有较强的污染物降解能力的不动杆菌(Acinetobacter)相关序列在Sul-1土壤细菌群落中被发现。In order to investigate molecular diversity of bacteria community from both a normal arable soil (Nor-1)and an arsenic and sulphate polluted soil(Sul-1).Environmental total DNA was directly extracted from two soil samples.The 16S rRNA genes were amplified from the total DNA and construction a clone library.Positive clones were randomly selected from the library and identified by amplified ribosomal DNA restriction analysis(ARDRA)and sequencing,then constructed phylogenetic tree.23 unique clone sequences from Nor-1 soil were classified into 5 bacterial phylum including Acidobacteria(12.3％,8/65),Actinobacteria(3.1％,2/65),Firmicutes(21.5％,14/65),Nitrospira(3.1％,2/65)and Proteobacteria(60％,39/65),while 19 unique clone sequences from Sul-1 soil were classified into 2 bacterial phylum including Firmicutes(29.5％,13/44),and Proteobacteria(70.5％,31/44).The result suggested that the high concentration of arsenic and sulphate influenced the bacterial population of Sul-1 soil leading to construction of obviously specific bacteria community.Interestingly,a lot of Acinetobacter related sequences have been detected in Sul-1 soil bacteria community including clone Sul1 1/15,Su112/7 and Sul12/11.Because Acinetobacter strains are often ubiquitous,exhibit metabolic versatility,those related strains may be good targets for exploiting novel arsenic detoxification bacteria.

关 键 词：砷及硫酸盐污染土壤 16S RRNA基因 ARDRA 细菌多样性 

分 类 号：X53[环境科学与工程—环境工程] X172