HPCE法测定归脾丸中远志皂苷元和远志酸的含量  

HPCE determination of senegenin and polygalacic acid in Guipi pills

作　　者：王洁[1] 张一鸣[1] 郝增艳[1] 胡明勋[1] 陈安家[1] 

机构地区：[1]山西医科大学药学院,太原030001

出　　处：《药物分析杂志》2011年第10期1891-1893,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：山西省自然科学基金资助项目(2008011077-1)

摘　　要：目的:建立测定归脾丸中远志皂苷元和远志酸含量的高效毛细管电泳法。方法:采用未涂层毛细管柱(75μm×57 cm,有效长度50 cm),运行缓冲液为20 mmol.L-1硼砂溶液与20%甲醇(pH=8.0),分离电压25 kV,检测波长210 nm。结果:远志皂苷元、远志酸的线性范围分别为13.25～212μg.mL-1(r=0.9971)和12.5～200μg.mL-1(r=0.9977),平均回收率(n=5)分别为101.5%和100.4%。结论:本法简便、准确,重复性好,可用于归脾丸的质量控制。Objective：To develop a capillary electrophoresis method for determination of senegenin and polygalacic acid in Guipi pills.Methods：The separation was carried out on an uncoated fused silica capillary（75 μm×57 cm,effective length 50 cm）.The separation voltage was 25 kV.The running buffer was 20 mmol·L-1 Na2B4O7 containing 20% methanol（pH=8）,and the detection wavelength was 210 nm.Results：The linear ranges were 13.25-212 μg·mL-1（r=0.9971） for senegenin and 12.5-200 μg·mL-1（r=0.9977） for polygalacic acid.The average recoveries of senegenin and polygalacic acid in Guipi pills were 101.5% and 100.4%.Conclusion：This method is proved to be simple,rapid and accurate,and can be used as a new method for the quality control of Guipi pills.

关 键 词：远志皂苷元 远志酸 毛细管电泳 归脾丸 

分 类 号：R917[医药卫生—药物分析学]