HPLC法同时测定齿叶白鹃梅叶子中芹菜素-7-O-β-D-新橙皮糖苷和芹菜素-7-O-β-D-葡萄糖苷的含量  

HPLC determination of apigenin-7-O-β-D-neospheroside and apigenin-7-O-β-D-glucopyranoside in the leaves of Exochorda serratifolia S.Moore

作　　者：才谦[1] 王聪[2] 刘玉强[1] 

机构地区：[1]辽宁中医药大学,大连116600 [2]铁岭卫生职业学院,铁岭112008

出　　处：《药物分析杂志》2011年第10期1899-1901,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立同时测定齿叶白鹃梅叶子中芹菜素-7-O-β-D-新橙皮糖苷和芹菜素-7-O-β-D-葡萄糖苷含量的HPLC法。方法:采用Topsil C18(5μm,4.6 mm×250 mm)色谱柱,流动相为乙腈-2%醋酸水溶液(23∶77),流速1.0 mL.min-1,检测波长267 nm。结果:芹菜素-7-O-β-D-新橙皮糖苷和芹菜素-7-O-β-D-葡萄糖苷的线性范围分别为1.424～8.544μg(r=0.9996)和0.17～1.02μg(r=0.9995);平均回收率(n=6)分别为103.0%(RSD=2.2%)和100.4%(RSD=2.1%)。结论:该方法准确、可靠,可用于齿叶白鹃梅叶子的质量控制。Objective：To develop an HPLC method for determination of the contents of apigenin-7-O-β-D-neospheroside and apigenin-7-O-β-D-glucopyranoside in the leaves of Exochorda serratifolia S.Moore.Methods：Topsil C18（5 μm,4.6 mm×250 mm） column was adopted with a mobile phase of acetonitrile-2% acetic acid（23∶77）,at the flow rate of 1.0 mL·min-1.The UV detection was set at 267 nm.Results：The linear ranges of apigenin-7-O-β-D-neospheroside and apigenin-7-O-β-D-glucopyranoside were 1.424-8.544 μg（r=0.9996）和0.17-1.02 μg（r=0.9995）,respectively;The average recoveries of apigenin-7-O-β-D-neospheroside and apigenin-7-O-β-D-glucopyranoside were 103.0%（RSD=2.2%） and 100.4%（RSD=2.1%）.Conclusion：The method is accurate and reliable,and can be used to the quality control of leaves of Exochorda serratifolia.

关 键 词：齿叶白鹃梅 高效液相色谱法 黄酮成分 芹菜素新橙皮糖苷 芹菜素葡萄糖苷 

分 类 号：R917[医药卫生—药物分析学]