高效抗肿瘤抗生素卡里奇霉素的分离纯化与鉴定  

Purification and identification of a high active anti-tumor antibiotic calicheamicin

作　　者：陈华 郑玲辉 孟强 王继栋 

机构地区：[1]浙江海正药业股份有限公司中研院微生物研究所,台州318000

出　　处：《中国抗生素杂志》2011年第10期758-761,共4页Chinese Journal of Antibiotics

摘　　要：目的研究从一株棘孢小单孢菌高产突变株912-36-2(Micromonospora echinospora 912-36-2)的发酵液中分离纯化抗肿瘤抗生素卡里奇霉素(calicheamicin)的方法,并得到纯品。方法发酵液通过有机溶剂萃取、硅胶分离、制备HPLC精制等方法分离纯化卡里奇霉素,并用LC-MS方法鉴定卡里奇霉素的各组分。结果从M.echinospora 912-36-2发酵液中分离出一个卡里奇霉素组分,LC-MS检测结果证明其分子量为1367Da,为目标活性组分γ1I。结论通过建立卡里奇霉素的分离提取工艺,为该产品的产业化开发提供基础。Objective Calicheamicin is a high active antitumor antibiotic produced by Micromonospora echinospora. The strain 912-36-2 is a high-yielding mutant from original strain by NTC and microwave mutation. The aims of this study were to purify calieheamicin from culture broth of M. echinospora 912-36-2. Methods The culture broth was extracted with the same volume of ethyl acetate. The organic extract was evaporated and the residues were added with hexane. The hexane insoluble was collected by filtration. The insoluble was extracted by ethyl acetate and the extract was precipitated by a mixture of diethyl ether and hexane. The crude calicheamicin was obtained after filtration. Then calicheamicin was further purified by silica gel chromatography and preparative HPLC. The calicheamicin homologues produced by M. echinospora 912-36-2 were analyzed by LC-MS. Results The purified calicheamicin was obtained after the isolation steps. The compound was analyzed by LC-MS and was identified as calicheamicin ~,~ with molecular weight of 1367Da. Conclusion The established purification steps were useful for further industrialization of this product.

关 键 词：抗肿瘤抗生素 卡里奇霉素 分离纯化 鉴定 

分 类 号：Q936[生物学—微生物学]