离体条件下药用菊花不同品种羟脯氨酸耐受性比较研究  

Study on hydroxyproline tolerance of different cultivars of Chrysanthemum morifolium in vitro

作　　者：朱艳芳[1] 郭朝阳[1] 薛建平[1] 张爱民[1] 盛玮[1] 宋运贤[1] 

机构地区：[1]淮北师范大学生命科学学院资源植物生物学安徽省重点实验室,安徽淮北235000

出　　处：《中国中药杂志》2011年第19期2625-2628,共4页China Journal of Chinese Materia Medica

基　　金：安徽省自然科学基金项目(090413252);安徽高校省级自然科学研究重点项目(KJ2009A160)

摘　　要：目的:比较5种药用菊花试管苗在离体条件下对羟脯氨酸的耐受性,为药用菊花引种栽培和育种提供依据。方法:以5种药用菊花试管苗为材料,接种于添加不同浓度羟脯氨酸的培养基中培养,测定植株叶片内游离脯氨酸的含量,并统计植株的受害指数和存活率。结果和结论:在高浓度羟脯氨酸胁迫下,亳菊和怀菊试管苗受害指数较低,成活率相对较高,叶片内游离脯氨酸含量高,具有较强的HYP耐受性。滁菊和贡菊植株存活率低于亳菊和怀菊,表现为中等的HYP耐受性。杭菊试管苗存活率最低,HYP耐受性最差。Objective： To investigate hydroxyproline tolerance of Chrysanthemum morifolium plantlets included ＂ Boju＂, ＂Huaiju＂ , ＂Chuju＂ , ＂Gongju＂ and ＂Hangju＂ ,and provide references basis for excellent cultivar and breeding of Ch. morifolium. Method： Plantlets in vitro from five kinds of Ch. morifolium were inoculated on medium added with different concentrations of hydroxyproline. Free proline in leaves from plantlets was determined, then the damage index and survival rate were compared. Result and Conclusion： The results showed that hydroxyproline tolerance of ＂Boju＂ and ＂ Huaiju＂ were superior, the survival rates and free proline of them were higher, but the damage index was inferior. The hydroxyproline tolerance of ＂ Hangju＂ was the worst, and the survival rate was minimum. The survival rate of ＂Chuju＂ and ＂ Gongju＂ was between ＂Boju＂ and ＂Hangju＂, and the hydroxyproline tol- erance of them was also medium.

关 键 词：药用菊花 离体培养 胁迫筛选 羟脯氨酸 

分 类 号：S567.239[农业科学—中草药栽培]