树豆菧类化合物longistylin A和longistylin C的检测  

Determination of longistylin A and longistylin C in Cajanus cajan

作　　者：王达武[1] 肖敏勋[1] 李宜融[1,2] 沈小玲[1] 卢元媛[1] 刘抗伦[1] 李正红[3] 胡英杰[1] 

机构地区：[1]广州中医药大学热带医学研究所药学研究室,广东广州510405 [2]国立中医药研究所中药标本馆 [3]中国林业科学研究院资源昆虫研究所,云南昆明650224

出　　处：《中国中药杂志》2011年第19期2680-2683,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2009ZX09103-436)

摘　　要：目的:建立以药物活性和专属性菧类成分longistylin A与longistylin C为评价指标的树豆(木豆)药材质量控制方法。方法:采用硅胶柱色谱分离纯化有关成分,光谱技术鉴定化学结构;反相高效液相色谱法测定指标成分的含量。结果:从树豆叶分离鉴定了2种指标成分longistylin A和longistylin C;采用Thermo BDS Hypersil C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水(80∶20)为流动相可使指标成分获得良好分离;longistylin A和longistylin C分别在0.002 88～0.057 6μg和0.011 2～0.224μg呈良好的线性关系,平均回收率分别为98.9%,97.2%,RSD分别为2.4%,2.2%。结论:建立了以树豆叶中药物活性菧类成分为指标的药物分析方法,可用于树豆(木豆)药材的质量控制。Objective： To establish quality control criteria for medicinal herb Cajanus cajan based on the determination of longistylin A and longistylin C, two bioactive and specific stilbenes of the plant. Method： Longistylin A and longistylin C were obtained from the leaves of C. cajan by silica gel column chromatography and identified as marker compounds of this plant by spectroscopic analysis. A RP-HPLC method was established to determine the two compounds. Result： Longistylin A and longistylin C were well separated on a Thermo BDS Hypersil C zs column （4. 6 mm × 250 mm, 5μm） with a mobile phase methanol-water （8：2）, and showed good linearity in the range of 0. 002 88-0. 057 6 μg and 0. 011 2-0. 224 p,g, respectively. The average recoveries were 98.9% and 97.2% with RSD of 2. 4% and 2.2% for these two compounds, respectively. Conclusion ： The established analysis method is simple and accurate, whicn can be used for quality control of C. cajan.

关 键 词：树豆 longistylinA longistylinC 结构鉴定 高效液相色谱 

分 类 号：R284[医药卫生—中药学]