小檗碱的调脂作用与CPTⅠA基因表达的关系研究  

Study on effect of berberine on modulating lipid and CPTⅠA gene expression

作　　者：王红[1] 师凌云[1] 尹华峰[1] 周岐新[1] 

机构地区：[1]重庆医科大学药理教研室,重庆400016

出　　处：《中国中药杂志》2011年第19期2715-2718,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30572353)

摘　　要：目的:研究小檗碱对高脂血症大鼠的调脂作用及对肝脏细胞CPTⅠA基因表达的影响。方法:按血脂高低将大鼠随机分为正常组,高脂模型组,小檗碱3006,0 mg.kg-1.d-1组和洛伐他汀7.2 mg.kg-1.d-1组。正常组用基础饲料喂养,其余各组喂以高脂、高胆固醇饲料,制备实验性高脂血症模型。于给药12周,分别取血样,采用全自动生化分析仪测定血脂变化;以RT-PCR和Western blot法评价大鼠肝脏细胞CPTⅠA mRNA和蛋白表达以及PPARαmRNA表达。结果:与高脂模型组相比,小檗碱呈剂量依赖性明显阻遏总胆固醇(TC)及低密度脂蛋白胆固醇(LDL-C)水平的增加和提升高密度脂蛋白胆固醇(HDL-C)水平;明显促进CPTⅠA mRNA和蛋白表达,但对PPARαmRNA表达无明显影响;洛伐他汀也显著阻遏TC,TG,LDL-C水平增加和提升HDL-C水平,但对CPTⅠA和PPARα基因表达均无明显影响。结论:小檗碱具有良性调血脂作用,其作用机制可能涉及与促进脂质代谢相关的CPTⅠA基因表达有关。Objective： To investigate the modulating effect on lipid and gene expressions of CPT I A caused by berberine （Ber） in experimental hyperlipidemia rats. Method： Male SD rats were randomly divided into 5 groups according to the blood lipid values： normal group, hyperlipidemia group, 300 mg·k-1·d-1 Ber-treated group, 60 mg·k-1·d-1Ber-treated group,and 7. 2 mg·k-1·d-1 lovastatin-treated group. Normal group were fed with base diet and other groups were fed with high fat and cholesterol diet. 12 weeks after drugs were given the TC, TG, LDL-C, and HDL-C from rat blood samples were tested by automatic biochemistry analyzer. Gene expressions of CPT I A and PPARu were evaluated by RT-PCR and Western blot, respectively. Result： It was shown that Ber significantly decreased TC and LI）L-C, but increased HDL-C in dose-dependent manner, elevated expressions of CPT I A mR- NA and protein without influence on PPARα expression. Similar effects from lovastatin on lipidemia were observed except the Ber effect on CPT I A gene expression. Conclusion： Ber has modulating effect on the lipid metabolism, the mechanism of which may be by pro- moting the CPT I A gene expression.

关 键 词：小檗碱 高脂血症 CPTⅠA PPARΑ 脂代谢 

分 类 号：R285[医药卫生—中药学]