基于聚硫堇和层层组装碳纳米管/HRP的过氧化氢传感器研究  

H2O2 Biosensor Based on Poly(thionine) and Layer-by-Layer Self-assembly of Carbon Nanotubes and HRP

作　　者：杨绍明[1] 黄爱花[1] 查文玲[1] 魏志鹏[1] 郑龙珍[1] 

机构地区：[1]华东交通大学基础学院化学化工系,南昌330013

出　　处：《化学学报》2011年第18期2143-2147,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(Nos.21065004;20965003);江西省自然科学基金(No.2009GQH0022)资助项目

摘　　要：将电聚合和层层组装方法有效结合构筑了聚硫堇(PTH)电子介体修饰的碳纳米管(CNTs)/辣根过氧化物酶(HRP)多层膜无试剂H2O2传感器.利用电化学阻抗谱对CNTs/HRP多层膜的组装过程进行监测,用循环伏安法和计时电流法研究了该HRP电极的电化学行为.探讨了酶组装层数、工作电位、pH值和碳纳米管对电极响应的影响.该传感器对过氧化氢在2.0×10-6～6.29×10-3 mol/L范围内呈良好的线性响应,相关系数为0.998,检测限为1.0×10-6 mol/L.Carbon nanotubes（CNTs）/horseradish peroxidase（HRP） multilayer reagentless H2O2 biosensor based on poly（thionine）（PTH） mediator was fabricated by the combination of electro-polymerization and layer-by-layer self-assembly method.Electrochemical impedance spectroscopy was used to monitor the layer-by-layer self-assembly process of CNTs/HRP multilayer.The electrochemical behavior of the HRP biosensor was studied by the cyclic voltammetry（CV） and chronoamperometry（I-t）.The effect of the num-ber of assembled enzyme layers,work potential,pH value and CNTs were investigated on the response of HRP electrode.The biosensor shows a good linear range of 2.0×10-6～6.29×10-3 mol/L for H2O2,R=0.998,with a detection limit of 1.0×10-6 mol/L.

关 键 词：聚硫堇 电聚合 层层组装 传感器 过氧化氢 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]