体内动员及诱导骨髓干细胞治疗大鼠糖尿病的疗效  

Therapeutic Effects of In Vivo Mobilizing and Inducing Bone Marrow Stem Cells on Diabetes of Rats

作　　者：许忆峰[1] 赵宇航[1] 袁凤山[1] 

机构地区：[1]中国医科大学附属第一医院内分泌科,沈阳110001

出　　处：《中国医科大学学报》2011年第10期881-884,共4页Journal of China Medical University

基　　金：辽宁省教育厅高校科研计划(05L573)

摘　　要：目的联合应用表皮生长因子(EGF)与促肝细胞生长因子(pHGF)体内诱导动员后的自体骨髓干细胞,探讨能否使其转分化为胰岛素分泌细胞,观察该方法对链脲佐菌素(STZ)大鼠糖尿病的治疗作用。方法 40只SD大鼠随机分为对照组、糖尿病组、动员组及诱导组,每组10只,后3组均用STZ制做糖尿病大鼠模型,成模后动员组和诱导组皮下注射重组人粒细胞集落刺激因子10μg.kg-.1d-1,连续15 d,对照组和糖尿病组注射生理盐水。诱导组于动员第2日腹腔注射EGF 1μg.kg-.1d-1、pHGF0.3 mg.kg-.1d-1,连续14 d;其他3组注射生理盐水对照。监测大鼠血糖、胰岛素水平,实验结束时免疫组化染色并计算胰腺中胰岛素阳性细胞百分比。结果治疗后诱导组大鼠血糖明显低于糖尿病组及动员组(P<0.05),血清胰岛素明显高于另外2组(P<0.05),胰腺中胰岛素阳性细胞百分比明显高于另外2组(P<0.01)。结论 EGF联合pHGF体内可诱导动员后自体骨髓干细胞转分化为胰岛素分泌细胞,可以增加血浆胰岛素水平,降低血糖,治疗大鼠糖尿病。Objective To explore the possibility of mobilized bone marrow stem cells （BMSCs） induced by epidermal growth factor（EGF） and hepatocyte growth-promoting factor （pHGF） in vivo differentiating into insulin-producing cells and to test the therapeutic potency of insulin-producing cells （IPCs） on diabetes of rats. Methods 40 SD rats were randomly divided into four groups which were normal control group,untreated diabetes group,mobilization group and induction group with 10 rats in each group. Rats in the latter three groups received intraperitoneal injection of streptozotocin （STZ） to establish diabetes model. Rats in mobilization group and induction group were treated with subcutaneously injection of recombination human granulocyte colony stimulating factor （rhG-CSF, 10 μg·kg^-1·d^-1） once a day for 15 days. Rats in induction group were treated with intraperitoneal injection of EGF （1 μg·kg^-1·d^-1） and pHGF（0.3 mg·kg^-1·d^-1） once a day for 14 days the day after mobilization injection. Before and after experiment blood glucose and serum insulin were measured, and insulin-positive cells in pancreas were detected by immtmohistochemistry after experiment. Results Blood glucose level of induction group was significantly lower than that of the untreated diabetes group and mobilization group （P 〈 0.05 ） while serum insulin was significantly higher （P 〈 0.05 ）. There were significantly more pancreatic insulin-positive cells in induction group than the untreated diabetes group and mobilization group （P 〈 0.01 ）. Conclusion Combination of EGF with pHGF after mobilization could induce bone marrow stem cells differentiating into insulin- secreting cells in streptozotocin-induced diabetic rats and improve hyperglycemia.

关 键 词：骨髓干细胞 糖尿病 重组人粒细胞集落刺激因子 表皮生长因子 促肝细胞生长因子 

分 类 号：R587.1[医药卫生—内分泌]