橄榄叶提取物对大鼠急性炎症和痛觉过敏的研究  

Effects of OLE on Carrageenan-induced Acute Inflammation and Hyperalgesia in Rats

作　　者：耿成燕[1] 宫德正[1] 姜丽平[1] 曹军[1] Hiroyuki Yoshimura 仲来福[1] 

机构地区：[1]大连医科大学中日合作医药科学研究所,大连116044 [2]日本卫材株式会社食品与化学部

出　　处：《天然产物研究与开发》2011年第5期931-934,共4页Natural Product Research and Development

摘　　要：本文采用鹿角菜胶诱导的大鼠急性炎症模型观察橄榄叶提取物(OLE)的抗炎与镇痛作用及对致炎因子TNF-α和IL-1β的mRNA表达。用鹿角菜胶注射到大鼠右后足内,分别于鹿角菜胶注射前30 min和注射后120 min灌胃给予OLE 100、250和500 mg/kg体重。鹿角菜胶处理后测量足跖肿胀度及痛域。用RT-PCR检测足跖垫内组织TNF-α、IL-1β和IL-10的mRNA表达。结果显示,经灌胃给予OLE100、250和500 mg/kg体重,均能明显降低大鼠后足肿胀度,增加痛域,并明显降低致炎因子TNF-α和IL-1β的mRNA表达。本研究提示OLE具有抗炎和镇痛作用,其作用机制可能与抑制TNF-α和IL-1β的mRNA表达有关。The aim of this study was to assess the inhibitory role of olive leaves extract （OLE） on carrageenan-induced swelling and hyperalgesia of rat paw. Paw inflammation was assessed by the increase in paw volume and hyperalgesia. The levels of the tumor necrosis factor alpha （TNF-α） ,interleukin 1/3 （IL-1β） and interleukin 10 （IL-10） mRNA in the tissue of right paw were estimated by reverse transcription-polymerase chain reaction （RT-PCR）. The results showed that the paw pressure thresholds of rats orally administered OLE significantly increased the degree swelling of right hind paw showed a statistically significant reduction, compared with rats in model control. OLE decreased expression of pro-inflammatory cytokines IL-1β and TNF-α. mRNA The results of the study suggest that OLE could have anti-inflammatory and anti-hyperalgesic effects and the mechanisms of these effects may be involved in inhibition of TNF-α and IL-1β mRNA expression.

关 键 词：橄榄叶提取物 痛域 肿胀度 鹿角菜胶 炎症 大鼠 

分 类 号：R285.5[医药卫生—中药学]