紫叶李树叶中山奈酚的提取工艺研究  

Optimum Extraction Technology of Kaempferol in Prunus cerasifera Leaves

作　　者：吴波[1] 张维农[2] 严建芳[3] 张寒俊[1] 

机构地区：[1]武汉工业学院分析测试中心 [2]武汉工业学院食品科学与工程学院 [3]武汉工业学院化学与环境工程学院,武汉430023

出　　处：《天然产物研究与开发》2011年第5期952-955,共4页Natural Product Research and Development

基　　金：湖北省教育厅重点科研资助项目(D20101712)

摘　　要：该文测定了紫叶李树叶中各黄酮苷元的含量,并对其中山奈酚的提取工艺进行了研究,重点探讨了采用乙醇法提取紫叶李树叶中山奈酚的最佳工艺条件,试验结果表明:紫叶李树叶中各黄酮苷元主要是槲皮素和山奈酚,乙醇提取紫叶李树叶中山奈酚的最佳工艺参数为浸提剂乙醇浓度为70%﹑浸提温度为50℃、料液比为1∶15、浸取时间为1.5 h。紫叶李树叶中山奈酚的浸取率为92.7%,经浸取、浓缩、水解、萃取、结晶工艺后,其紫叶李树叶中山奈酚的总得率为56.6%,山奈酚的纯度为90.6%。The content of flavonoids in Prunus cerasifera leaves and its extraction technology were studied in this paper. By means of ethanol extracting,the optimum extraction conditions of kaempferol in P. cerasifera leaves were determined. The results showed that the content of quercetin and kaempferol were major fiavonoids in P. cerasifera leaves, the optimum conditions of ethanol extracting were obtained as follows ： 70% ethanol as extraction solvent at 50 ℃ for 1.5 h, and the ratio of material to solvent was 1： 15. Using the technological parameters above mentioned, the average extraction rate of kaempferol was 92.7 %. After extracting, concentration, hydrolyzing, extraction and crystal, the average recoveries and product purity of kaempferol were 56.6% and 90.6% ,respectively.

关 键 词：紫叶李树叶 山奈酚 提取工艺 

分 类 号：Q946.91[生物学—植物学] R284.2[医药卫生—中药学]