电针对海洛因成瘾大鼠学习记忆及海马CREB表达的影响  

The effect of acupuncture on learning and memory in heroin-addict rats and on expression of CREB in hippocampus

作　　者：刘慧英[1] 潘贵书[1] 

机构地区：[1]遵义医学院生理教研室,贵州遵义563003

出　　处：《癫痫与神经电生理学杂志》2011年第5期263-268,共6页Journal of Epileptology and Electroneurophysiology(China)

基　　金：贵州省省长基金资助（黔科教办[2007]03号）

摘　　要：目的：探讨电针对海洛因成瘾大鼠学习记忆的影响及作用机制。方法130只Wistar大鼠随机分为对照组、海洛因成瘾组（简称成瘾组）和海洛因成瘾后作电针治疗组（简称电针组）。进行跳台实验测试大鼠学习记忆能力，并用免疫组化方法检测鼠脑海马环磷酸腺苷反应元件结合蛋白（CREB）和磷酸化CREP（P—CREB）的表达。结果：成瘾组大鼠学习记忆下降（P％0．01），电针组大鼠学习记忆有所改善（P〈0．01）；成瘾组大鼠海马CREB、P—CREB表达较对照组增高（P〈0．05），电针组更高（P〈0．01）。结论：电针治疗可在一定程度上对抗海洛因成瘾大鼠所致的学习记忆下降，其机制可能与海马内CREB、P—CREB表达变化有关；CREB、PCREB参与学习记忆及海洛因成瘾的形成。Objective：To observe the effects of electroacupuncture on the learning and memory in heroiwaddict rats and investigate its potential mechanism. Methods：Thirty Wistar rats were randomly divided into the control group, the heroin-addict group and the acupuncture group. The 3 groups of rats were respectively conducted in step down tests to detect the learning and memory of rats. The expression of CREB and p-CREB in hippocampus were detected by immunohisto chemistry. Results：The dependence of learning and memory in the heroin-addict group were reduced （P〈0.01）. Electroacupuncture improve learning and memory of rats （P〈0.01） ; the expression of CREB and p-CREB in hippocampus in the her oin-addict group was more than that of the control group （P〈0.05） ; In the electro acupuncture group, the expression of CREB and p-CREB in hippocampus was more than that of the control group （P〈0.01）. Conclusion： Heroin-addict can damage the ability of learning and memory of Wistar rats. To a cer tain extent, electro-acupuncture can combat it. Its mechanism may be related to CREB, p-CREB expression in hippocampus; CREB and p-CREB involved in learning,memory and addiction memory formation.

关 键 词：电针 海洛因成瘾 学习记忆 环磷酸腺苷反应元件结合蛋白(CREB) 磷酸化环磷酸腺苷反应元件结合蛋白(p—CREB) 

分 类 号：R595.5[医药卫生—内科学]