CP-154,526和verapamil对促肾上腺皮质激素释放因子分泌水平的影响  

Effects of CP-154,526 and Verapamil on Corticotrophin Releasing Factor Secreting Levels

作　　者：董静[1] 祝益民[1] 周文武[1] 袁发[1] 张川[1] 董春艳[1] 肖政辉[1] 桂永浩[2] 陈超[2] 

机构地区：[1]湖南省儿童医院,长沙410007 [2]复旦大学儿科医院,上海201102

出　　处：《医学研究杂志》2011年第9期66-68,共3页Journal of Medical Research

基　　金：湖南省科技厅基金资助项目(2008RS4029)

摘　　要：目的探讨促肾上腺皮质激素释放因子Ⅰ型受体(CRF-R1)拮抗剂CP-154,526(CP)和钙离子通道拮抗剂vera-pamil(VP)对幼年大鼠在缺氧缺血(HI)后并放置1天后血浆中促肾上腺皮质激素释放因子分泌水平的影响。方法 80只幼年大鼠随机分成8组,即对照组、假手术组、CP对照组、VP对照组、模型组(HI组)、HI+CP组、HI+VP组和HI+CP+VP组。用放射免疫法测定各组幼年大鼠血浆中CRF水平。结果与对照组比较,HI组、HI+CP组和HI+CP+VP组血浆CRF水平都显著降低(P<0.001、P<0.001、P<0.001);与假手术组比较,HI组、HI+CP组和HI+CP+VP组血浆CRF水平都显著降低(P<0.001、P<0.001、P<0.001);与CP对照组比较,HI组、HI+CP组和HI+CP+VP组血浆CRF水平都显著降低(P<0.001、P<0.001、P<0.001);与VP对照组比较,HI组、HI+CP组和HI+CP+VP血浆CRF水平都显著降低(P<0.001、P<0.001、P<0.001);与HI组比较,HI+CP组和HI+VP组血浆CRF水平显著增加(P<0.05、P<0.001);与HI+CP比较,HI+VP组血浆CRF水平显著增加(P<0.001);与HI+VP组比较,HI+CP+VP组血浆CRF水平显著降低(P<0.001)。结论幼年大鼠在HI后并放置1天后血浆CRF水平都显著减少;可是,当幼年大鼠用CP或者VP预处理后,血浆CRF分泌水平在缺氧缺血下的减少能被逆转;当用CP和VP同时预处理后,血浆CRF分泌水平在缺氧缺血下的减少没有被改变。Objective To explore the effects of CRF acceptor 1 （ CRF - R1） antagonist CP - 154,526 （CP） and Ca2 ＋ pathway an- tagonist verapamil （VP） on plasma CRF secreting levels in the young rats after hypoxic - isehemia （HI） and after the rats were placed for one day. Methods Eighty young rats were randomly divided into eight groups,which were the control groups,sham operated groups, CP control groups,VP control groups,model groups （ HI groups） ,HI ＋ CP groups,HI ＋ VP groups and HI ＋ CP ＋ VP groups. Plasma CRF lev- els of the young rats in all groups were measured by radioimmunoassay. Results Compared with the control groups,plasma CRF levels in the HI groups, HI ＋ CP groups and HI ＋ CP ＋ VP groups all significantly decreased （ P 〈 0. 001, P 〈 0.001, P 〈 0. 001 ）. Compared with the sham operated groups, plasma CRF levels in the HI groups, HI ＋ CP groups and HI ＋ CP ＋ VP groups all significantly decreased （ P 〈 0. 001 ,P 〈0. 001 ,P 〈0. 001 ）. Compared with the CP control groups,plasma CRF levels in the HI groups,HI ＋ CP groups and HI ＋ CP ＋ VP groups all significantly decreased （P 〈 0. 001 ,P 〈 0. 001 ,P 〈 0. 001 ）. Compared with the VP control groups, plasma CRF levels in the HI groups ,HI ＋ CP groups and HI ＋ CP ＋ VP groups all significantly decreased （P 〈 0. 001 ,P 〈 0. 001 ,P 〈 0. 001 ）. Compared with the HI groups, plasma CRF levels in the HI ＋ CP groups and HI ＋ VP groups all significantly increased （ P 〈 0.05, P 〈 0. 001 ）. Compared with the HI ＋ CP groups,plasma CRF levels in the HI ＋ VP groups significantly increased （P 〈 0. 001 ）. Compared with the HI ＋ VP groups, plasma CRF levels in the HI ＋ CP ＋ VP groups significantly decreased （ P 〈 O. 001 ）. Conclusion Plasma CRF levels in the young rats af- ter HI and after the young rats were placed for one day significantly decreased. But,after the young rats were pretreated with CP or VP,the decrease of plasma CRF secreting levels under HI can

关 键 词：缺氧缺血脑损伤促肾上腺皮质激素释放因子 CP-154 526 Verapamil幼年SD大鼠 

分 类 号：R995[医药卫生—毒理学]