新疆和田维吾尔族长寿老人永生细胞株的建立  

Establishment of Immortalized B Lymphoblast Cell Lines from the Longevous Uygur People in HeTian XinJiang

作　　者：袁晓华[1] 吐尔洪.克维尔 穆斯塔帕[2] 左宏莉[1] 乔艳辉[1] 郭伟鹏[1] 

机构地区：[1]乌鲁木齐市血液中心,830000 [2]新疆农业科学院微生物研究所

出　　处：《医学研究杂志》2011年第9期81-83,共3页Journal of Medical Research

基　　金：乌鲁木齐市科技局人才工程项目编号(P09131005)

摘　　要：目的通过用EB病毒转化外周血B淋巴细胞使之成为永生细胞株的方法,建立新疆和田维吾尔族长寿老人永生细胞株,为永久保存新疆长寿老人的基因遗传资源及后续研究奠定基础。方法常规制备EB病毒上清,用环孢素法和冻存全血法转化外周血B淋巴细胞。结果以环孢素法成功建立了5株新疆和田维吾尔族长寿老人永生细胞,其中百岁2人,90～99岁3人,建系成功率为100%(5/5),以冻存全血法建系成功1例(1/5)。HLA基因分型结果在建系前后完全一致。结论与冻存全血法相比,环孢素法能更有效地转化长寿老人永生细胞株,可为长寿的分子遗传机制研究以及某些细胞生物学性状的检测提供充足的实验材料。Objective To establish immortal cell lines from the longcvous Uygur people in HeTian. Methods We used two methods of transforming human B lymphocyte into immortal lymphoblast cell lines ： cyclosporinc method and frozen whole blood method. Results Both of the methods can be used to establish the ceil lines successfully. Cyclosporine method （5/5） was more efficient than frozen whole blood method （1/5）. Five immortal longevity cell lines were obtained successfully,which included 2 centenarians and 3 nonagenarians. The results of HLA genotyping showed the genetic stability of these immortal cell lines. Conclusion These immortal B lymphoblast cell lines would preserve the unique genome of longevous Uygur people in Hetian. It could provide adequate experimental material for the explo- ration of the origin and molecular mechanism of human longevity.

关 键 词：EB病毒 永生细胞株 维吾尔族长寿老人 和田 

分 类 号：R161.7[医药卫生—公共卫生与预防医学]