黔北一新鱼种遵义小钝吻鮠线粒体DNA的分离纯化  

Isolation and Purification of Mifochondrial DNA of New Species of Leioxassis Bleeker in Northern Guizhou

作　　者：肖贵榜[1,2] 葛正龙[2] 张艳磊[2] 李作祥[3] 

机构地区：[1]遵义职业技术学院,贵州遵义563000 [2]遵义医学院生物化学与分子生物学教研室 [3]贵阳职业技术学院生物化学工程系

出　　处：《现代农业科技》2011年第19期325-326,共2页Modern Agricultural Science and Technology

摘　　要：用差速离心法和改进的碱裂解法分离纯化遵义小钝吻鮠线粒体DNA,紫外检测其OD260/OD280在1.78～1.85,并计算出该鱼肝组织线粒体DNA的含量为0.613μg/g。纯化样品经紫外分析仪在200～290 nm波长范围内扫描,呈现典型的DNA吸收峰,其最大吸收峰在259～260 nm。以λDNA/HindⅢ的完全酶切片段作为相对分子量标准,利用电泳迁移率与分子量的对数的线性关系,由已知片段大小推算出遵义小钝吻鮠mtDNA分子大小为(15.44±0.16)kb。The mitochondrial DNA（mtDNA）from liver ofLeioxassis^ticrocrassirostris ZunyiensisXiao sp.nov.was isolated and purified by means of differential centrifugation and the improved alkaline lysisoThe OD260/OD280 was 1.78-1.85 by UV spectrophotometer,and the quality of mtDNA obtained was 0.613 μg/goUnder the wavelength scan of 200-290 nm by UV solution ,the Purification of DNA of the sample presented characteristic DNA absorption peaks and the maximum one in 259-260 nmoUsekDNA/Hind m restriction fragments as a relatively molecular weight standard, according to the electrephoretic migration rate was linearly related to the molecular weight, the mtDNA size was （15.44±0.16） kb in the fish .

关 键 词：遵义小钝吻鮠 线粒体DNA 分离纯化 黔北地区 

分 类 号：S917[农业科学—水产科学] Q523[生物学—生物化学]