病理性瘢痕与正常皮肤的蛋白质组学研究  

Proteomic analysis between pathological scars and normal skin

作　　者：胡志成[1] 朱家源[1] 朱斌[1] 郭栋[1,2] 陈斌[1] 张凯[1] 胡坤华[3] 黎明涛[3] 唐冰[1] 

机构地区：[1]中山大学附属第一医院烧伤外科,广东广州510080 [2]中山大学附属第三医院整形外科,广东广州510630 [3]中山大学中山医学院蛋白质组学研究中心,广东广州510080

出　　处：《中国病理生理杂志》2011年第9期1802-1806,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30973128);广东省自然科学基金资助项目(No.8151008901000082);广东省自然科学基金资助项目(No.2007B031504003)

摘　　要：目的:通过比较病理性瘢痕与正常皮肤组织的蛋白质组表达差异,寻找和筛选出病理性瘢痕形成机制中的特异蛋白质。方法:运用蛋白质组学技术,对8例瘢痕疙瘩、8例增生性瘢痕患者的组织和3例正常皮肤组织进行差异双向电泳(2D-DIGE),选择差异蛋白质斑点,进行MALDI-TOF/TOF质谱分析和生物信息学分析。结果:成功建立病理性瘢痕和正常皮肤组织的双向凝胶电泳图谱,瘢痕疙瘩、增生性瘢痕和正常皮肤组织凝胶电泳图谱中平均蛋白质斑点数分别为2 978、2 975和3 053。与正常皮肤组织相比,我们对瘢痕疙瘩和增生性瘢痕中差异超过4倍的斑点进行质谱分析和数据库检索,共鉴定出36种不同蛋白,包括:瘢痕疙瘩和增生性瘢痕表达相同的16种蛋白,其中上调蛋白8种,下调蛋白8种;只在瘢痕疙瘩中表达的有11种不同蛋白,其中上调9种,下调2种;只在增生性瘢痕中表达的有9种不同蛋白,其中上调4种,下调5种。结论:蛋白质组学较好地显示了病理性瘢痕与正常皮肤组织间的蛋白质表达差异;进一步研究鉴定出的差异蛋白质,有可能对揭示病理性瘢痕形成机制提供新的思路。AIM： To investigate and screen the sensitive proteins in the formation mechanism of p athological scars by comparing the results of differential proteomic analysis be tween pathological scars and normal skin.METHODS： Two-dimensional gel electrophoresis was used to detect the protein expression pr ofiles in 8 keloid patients,8 hypertrophic scar patients and 3 matched normal s kin patients.The proteins that showed differential expression of over 4-fold ch ange were cut and analyzed by MALDI-TOF/TOF mass spectrometry. RESULTS： A two-dimensional protein profiling comparison between pathological scars and no rmal skin was successfully established.On average,2 978 spots in keloid,2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin,there were totally 36 differe ntially-expressed proteins in keloid and hypertrophic scar identified from the s pots of over 4-fold change,including 16 proteins in both keloid and hypertrophi c scar（8 up-regulated and 8 down-regulated）,11 only in keloid（9 up-regulated and 2 down-regulated） and 9 only in hypertrophic scar（4 up-regulated and 5 down-regulated）. CONCLUSION： Proteomic analysis can identify the proteins with va riance of pathological scars versus normal skin,thus providing probable new clu es to reveal the formation mechanism of pathological scars.

关 键 词：瘢痕疙瘩 瘢痕 增生性 蛋白质组学 波形蛋白 Asporin OSTEOGLYCIN 

分 类 号：R619.6[医药卫生—外科学]