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作 者:王强[1,2] 汪光蓉[1,2] 凡瞿明[2] 赵明才[1,2] 唐中[1,2] 何兰[2] 张国元[1,2]
机构地区:[1]川北医学院医学检验系 [2]川北医学院附属医院检验科,四川南充637000
出 处:《第三军医大学学报》2011年第19期2058-2060,共3页Journal of Third Military Medical University
摘 要:目的通过对乙型肝炎病毒核酸相关抗原(hepatitis B virus nucleic acid related antigen,HBV NRAg)与HBeAg和乙型肝炎病毒DNA(hepatitis B virus DNA,HBV DNA)之间的比较,探讨HBV NRAg在评价病毒复制中的应用价值。方法收集157例HBsAg阳性血清,采用酶联免疫吸附试验(ELISA)、化学发光微粒子免疫测定(CMIA)和荧光定量PCR法分别对血清进行HBV NRAg、HBeAg和HBV DNA含量检测。同时对188例HBsAg阴性血清进行HBV NRAg特异性检测。结果 157例HBsAg阳性血清中,HBV NRAg,HBeAg和HBV DNA阳性率分别为75.79%(119/157)、37.57%(59/157)和51.59%(81/157),HBV NRAg与HBeAg和HBV DNA比较,差别有统计学意义(χ2=19.88,P=0.000;χ2=46.69,P=0.000)。分别在HBeAg和HBV DNA阳性病例中,HBV NRAg S/CO值与HBeAg S/CO值和HBV DNA基因组拷贝数呈正相关(r=0.506,P=0.000;r=0.499,P=0.000)。在一致性评价中,HBV NRAg同HBeAg和HBV DNA两个指标联合用于评价病毒复制的一致性较好(Kappa=0.52),其阳性符合率为94.79%,总符合率为78.98%。HBV NRAgELISA检测特异性为99.40%。结论 HBV NRAg可作为临床评价HBV复制以及乙肝治疗效果监测的初筛指标,适合在各个层次的医院开展检测。Objective To evaluate the correlations of hepatitis B virus nucleic acid related antigen(HBV NRAg),HBeAg and HBV DNA in HBsAg positive patients and to investigate the value of HBV NRAg in evaluating HBV replication.Methods A total of 157 serum samples were collected from HBsAg positive patients,and the levels of HBV NRAg,HBeAg and HBV DNA were measured by enzyme-linked immunosorbent assay(ELISA),chemiluminescent microparticle immunoassay(CMIA) and real-time PCR respectively.ELISA detection of HBV NRAg was also performed in 188 HBsAg-negative serum samples.Results In HBsAg-positive serum,the positive rates of HBV NRAg,HBeAg,and HBV DNA were 75.79%(119/157),37.57%(59/157),and 51.59%(81/157) respectively.HBV NRAg positive rate was significantly different as compared with HBeAg and HBV DNA(Chi square=19.88,P=0.000;Chi square=46.69,P=0.000).HBV NRAg S/CO value was positively correlated with HBeAg S/CO value and HBV DNA genomic copy number in HBeAg positive cases and HBV DNA positive cases,respectively(r=0.506,P=0.000;r=0.499,P=0.000).HBV NRAg as an evaluation index for virus replication was in good concordance with the combined indices of HBeAg and HBV DNA(Kappa=0.52).The positive coincidence rate was 94.79%,and the total coincidence rate was 78.98%.Test specificity of HBV NRAg ELISA was 99.40%.Conclusion HBV NRAg can be used as a preliminary screening index for clinical evaluation of HBV replication and monitoring of hepatitis B treatment outcome for hospitals at all levels.
关 键 词:乙型肝炎病毒核酸相关抗原 HBEAG HBV DNA HBV复制
分 类 号:R373.21[医药卫生—病原生物学] R446.6[医药卫生—基础医学]
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