HIV／HCV共感染者NK细胞数量和功能的研究  

Alterations of NK cell frequency and function in HIV/HCV co-infected patients

作　　者：张昕 赵鹏 康富标[2] 张新伟 司蝉 陈雪丽 陈威巍 赵敏 

机构地区：[1]解放军第三零二医院感染性疾病诊疗与研究中心,北京100039 [2]白求恩国际和平医院

出　　处：《中华实验和临床病毒学杂志》2011年第4期248-250,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：首都医学发展科研基金（课题编号2007-3036）;国家自然基金（课题编号81072423）

摘　　要：目的 研究HIV/HCV共感染者的NK细胞数量和功能的变化，探讨HIV/HCV共感染对NK细胞的影响。方法 应用流式细胞术（FCM）检测HIV/HCV共感染者（n=18）、单纯HIV感染者（n=20）、单纯HCV感染者（n=30）及健康对照（n=18）的NK细胞数量，用PMA和K562细胞刺激外周血单个核细胞（PBMC）、流式细胞术检测NK细胞分泌IFN-γ的能力，并用流式细胞毒性分析法检测NK细胞对K562细胞的杀伤效率，对各组NK细胞的功能进行分析和比较。结果 HIV/HCV共感染者、单纯HIV感染者、单纯HCV感染者的NK细胞频率、NK细胞分泌IFN-γ的能力以及对K562细胞的杀伤能力都显著低于健康对照；HIV/HCV共感染者的NK细胞绝对值和对K562细胞杀伤能力显著低于HIV和HCV单纯感染者。结论 HIV/HCV共感染对NK细胞数量和功能的影响较单纯HIV和HCV感染更加严重。Objective To study the impacts of HIV/HCV co-infection to NK cells by investigating the changes of frequencies and functions of NK cells in HIV/HCV co-infected patients. Methods Frequencies and counts of NK cells were measured in patients with HIV mono-infection, HCV monoinfection, HIV/HCV co-infection and health control （HC） group by flow cytometer （ FCM ）. After stimulated with PMA and K562 cells, PBMCs were examined the proportion of IFN-γ＋ NK cells by FCM. Proportion of killed K562 cells were detected to analyze the killing functions of NK cells. Results The frequencies of NK cells, the percentages of IFN-γ＋ NK cells as well as the functions of NK cells killing the K562 cells in HIV/ HCV co-infection, HIV mono-infection and HCV mono-infection groups were all lower than those of HC group significantly, the absolute counts and killing functions of NK cells in co-infection group were significantly lower than those of HIV or HCV mono-infection group. Conclusions The counts and functions of NK cells were affected more in HIV/HCV co-infections than those in HIV or HCV mono-infection.

关 键 词：HIV 肝炎病毒属 感染 杀伤细胞 天然 

分 类 号：R512.91[医药卫生—内科学]