恒河猴肠道腺病毒聚合酶基因片段的序列分析  

Sequencing and analyses of the adenovirus polymerase gene in fecal samples of captively bred rhesus macaques

作　　者：卢建溪[1] 曹红[2] 钱师宇[3] 王强[1] 李刚[2] 

机构地区：[1]中山大学附属第三医院疫苗研究所,510630 [2]中山大学附属第三医院感染科 [3]暨南大学医学院

出　　处：《中华实验和临床病毒学杂志》2011年第4期268-270,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家高技术研究发展计划（863计划）（No.2007AA021002）;广东省科技计划资助项目（No.20088030301101）

摘　　要：目的 研究非人灵长类动物猴腺病毒的分子多样性。方法 采集圈养的57只恒河猴粪便样本，以腺病毒聚合酶基因为目的基因，用PCR方法进行扩增，对PCR阳性产物进行克隆、测序，并进行系统进化分析。结果 57只恒河猴粪便样本有12份样本中存在腺病毒DNA，系统进化分析提示这些序列主要可分为两大组：类SAdV-6组（2个非重复序列）和类SAdV-7组（9个非重复序列）。此外，有三个克隆，其最相似序列分别为：SAdV-1,SAdV-3和HAdV-52。结论 我们的研究证实了腺病毒的确在非人灵长类粪便中普遍存在，揭示了本研究所涉及动物肠道内腺病毒的多样性以及系统进化情况。Objective In an attempt to study the moleculr characterization and epidemiology of simian adenoviruses in nonhuman primate （NHP） populations. Methods We examined a colony of captively bred rhesus macaques （Macaca mulatta） in China for the presence of adenoviral DNA in stool samples. This was done by using the PCR method that targeted the adenovirus polymerase gene, and the PCR positive fragments were cloned for sequencing and phylogenetic analyses. Results Among the 57 animals analyzed, fecal samples from 12 animals were positive for the presence of adenoviral DNA. The results suggested that the viral DNA clones were primarily segregated into two large groups： SAdV-6 （2 nonredundant sequences） and SAdV-7 （9 non-redundant sequences）. In addition, there were three clones with more similarity to SAdV-1, SAdV-3 and HAdV-52 respectively. Conclusion Our data confirmed the prevalence of adenoviral DNA in the feces of NHPs and revealed the heterogeneity and phylogenetics of the adenoviruses in the gastrointestinal tract of the study animals.

关 键 词：恒河猴 腺病毒 猴 基因 

分 类 号：S852[农业科学—基础兽医学]