免疫复合物型增效乙型肝炎疫苗细胞免疫检测方法的建立  

Establishment of cellular immunity of enhanced hepatitis B vaccine

作　　者：王娟[1] 吴刚[1] 赵莉[1] 李计来[1] 许丽锋[1] 徐静[1] 

机构地区：[1]北京生物制品研究所,100024

出　　处：《中华实验和临床病毒学杂志》2011年第4期310-312,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 建立免疫复合型增效乙型肝炎疫苗细胞免疫检测方法并与常规乙型肝炎疫苗进行比较。方法增效乙型肝炎疫苗肌内免疫BALB/c小鼠，Elispot方法检测小鼠脾脏淋巴细胞分泌IFN-γ的细胞频数（SFC），优化实验条件：免疫剂量、检测时间和特异性刺激物及刺激浓度。同时将增效乙型肝炎疫苗与常规乙型肝炎疫苗进行比较。结果5μg免疫组IFN-γ SFC明显高于2μg免疫组，初次免疫后3周IFN-γ SFC开始下降，多肽刺激IFN-γ SFC高于蛋白刺激，多肽浓度为2μg即可充分刺激斑点形成；增效乙肝疫苗免疫组IFN-γ SFC高于常规乙型肝炎疫苗组。结论 初步建立了免疫复合型增效乙型肝炎疫苗细胞免疫检测方法并证明其重复性良好，其细胞免疫水平高于常规乙型肝炎疫苗。Objective To establish the method to detect the cellular immune response of enhanced hepatitis B vaccine and make verification preliminary. Methods Immunized BALB/c mice with enhanced hepatitis B vaccine and detected the IFN-γ spots forming ceils （SFC） of mouse spleen cell by Elispot. Optimized the conditions of the experiment. Cellular immune response between enhanced hepatitis B vaccine and normal hepatitis B vaccine by Elispot were compared. Results IFN-γSFC was higher in 5μ g dose than in 2μg dose after immunization with enhanced hepatitis B vaccine and IFN-γ SFC was declined after immunization 3 weeks ago. IFN-γ SFC was higher in stimulus by peptide than by protein. Compared to normal hepatitis B vaccine, IFN-γ SFC was higher in enhanced hepatitis B vaccine. Conclusion Established the detection method to evaluate the cellular immunity of enhanced hepatitis B vaccine and tested the repeatability.

关 键 词：肝炎疫苗 乙型 酶试验 

分 类 号：R186[医药卫生—流行病学]