银屑病患者粒-单核巨噬细胞集落刺激因子表达的研究  

Expression of granulocyte macrophage-colony stimulating factor in patients with psoriasis

作　　者：谢欣 柴立 周毅成[2] 安娜 田静[2] 

机构地区：[1]黑龙江省皮肤病防治研究所,哈尔滨150001 [2]哈尔滨医科大学附属第一医院皮肤科

出　　处：《中华皮肤科杂志》2011年第10期731-732,共2页Chinese Journal of Dermatology

摘　　要：目的探讨脓疱性银屑病和寻常性银屑病患者皮损组织及外周血粒一单核巨噬细胞集落刺激因子（GM—CSF）表达的变化。方法免疫组化法和双抗体夹心酶联免疫吸附法（ELISA）分别检测脓疱性银屑病和寻常性银屑病患者皮损组织和外周血中GM—CSF表达水平，并与正常人进行比较。结果与正常人对照组相比，脓疱性银屑病组和寻常性银屑病组皮损组织中GM—CSF表达均增高（P〈0．01），且脓疱性银屑病组皮损组织中GM—CSF表达高于寻常性银屑病组（P〈0．01）。与正常人对照组相比，脓疱性银屑病组和寻常性银屑病组外周血中GM—CSF含量均升高（P〈0．01），且脓疱性银屑病组外周血中GM—CSF水平高于寻常性银屑病组（P〈0．01）。结论GM—CSF可能参与银屑病的发病。Objective To investigate the expression of granulocyte macrophage-colony stimulating factor （GM-CSF） in skin lesions and sera of patients with pustular psoriasis and psoriasis vulgaris. Methods Tissue specimens were obtained from the lesions of 15 patients with pustular psoriasis, 15 patients with psoriasis vulgaris and 15 normal human controls. Immunohistochemistry and dual antibody sandwich enzyme-linked immunosorbent assay （ELISA） were carried out to detect the levels of GM-CSF in the tissue and serum specimens from the patients and normal human controls, respectively. Results Significantly higher levels of GMCSF were observed in the tissue and serum specimens from patients with pustular psoriasis and psoriasis vulgaris compared with the normal controls （all P 〈 0.01 ）, as well as in those from the patients with pustular psoriasis compared with the patients with psoriasis vulgaris （both P 〈 0.01）. Conclusion GM-CSF may be involved in the pathogenesis of psoriasis.

关 键 词：单核巨噬细胞集落刺激因子 银屑病患者 因子表达 双抗体夹心酶联免疫吸附法 脓疱性银屑病 寻常性银屑病 皮损组织 CSF 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]