TLR3激动剂对B细胞功能的影响  

Effects of TLR3 agonist on B cell functions

作　　者：钱莉[1] 傅奕[2] 潘兴元[1] 田芳[1] 龚卫娟[1] 季明春[1] 

机构地区：[1]扬州大学医学院病原生物学与免疫学教研室,225001 [2]扬州大学医学院生物化学教研室,225001

出　　处：《免疫学杂志》2011年第10期833-836,共4页Immunological Journal

基　　金：国家自然科学基金(81001308;30800182);江苏省自然科学基金(BK2010315);扬州大学高层次人才科研启动资金(0474910015048)

摘　　要：目的观察TLR3激动剂Poly I:C对B细胞功能的影响,包括细胞增殖、共刺激分子表达、细胞因子和抗体的分泌情况。方法利用免疫磁珠法分选小鼠脾脏CD19+B细胞,RT-PCR法证实其表达TLR3。体外用Poly I:C刺激B细胞一定时间后,CFSE分裂法检测B细胞增殖,流式细胞术(flow cytometry,FCM)检测B细胞表面共刺激分子表达,CBA(cytometric bead ar-ray)法或ELISA法检测培养上清中细胞因子含量,CBA法检测B细胞分泌Ig的亚型。结果 Poly I:C可以促进B细胞增殖,上调B细胞表面CD40、CD80、CD86和MHC II类分子的表达,促进IL-6和TNF-α的高分泌,诱导IgG1κ抗体的产生。结论 Poly I:C可以通过促进增殖、细胞因子分泌,诱导抗体产生和上调共刺激分子表达等多方面调节B细胞功能。To explore the immunomodulatory effects of Poly I：C（TLR3 agonist） on B cells,freshly purified splenic B cells or CFSE-labeled B cells were cultured in presence or absence of PolyI：C.After 24 h,culture supernatants were collected for screening of cytokine secretion by ELISA or cytometric bead array（CBA） technology.At day 6 and 9,the dilution of CFSE was assayed by flow cytometry（FCM）;at day 2 and 5,cells were analyzed for costimulatory molecules expression by FCM.At day 5,culture supernatants were collected and assayed for immunoglobulin isotyping using the cytometric bead-based mouse Ig isotyping system by FCM.We found that B cells proliferate weakly in response to Poly I：C.Interestingly,Poly I：C led to increased the expression of CD40,CD80,CD86 and MHC class II,promoted IL-6 and TNF-α secretion,and induced IgG1 κ production by B cells.In conclusion,TLR3 agonist can regulate B cell function by promoting proliferation,cytokine secretion,IgG1 κ production and costimulatory molecules expression.

关 键 词：TLR3 B细胞 功能 

分 类 号：R392.1[医药卫生—免疫学]