人肠道正常粘膜组织与外周血中记忆性IL-22^+T淋巴细胞频率及表型的比较  

Comparison of the frequencies and phenotypes of IL-22-producing T cells from human normal membrane of intestinal tract and peripheral blood mononuclear cells

作　　者：王慧[1] 刘昀[1] 付笑迎[1] 兰平[2] 何晓生[2] 吴长有[1] 

机构地区：[1]中山大学医学院免疫研究所热带传染病教育部重点实验室,广州510080 [2]中山大学附属第六医院结直肠肛门外科

出　　处：《免疫学杂志》2011年第10期861-865,共5页Immunological Journal

基　　金：国家重点基础研究发展计划(973)资助项目(2007CB512404)

摘　　要：目的比较人肠道正常粘膜组织与外周血中IL-22+T淋巴细胞的频率及其表型特征。方法分离人肠道正常粘膜与外周血中单个核细胞,anti-CD3+anti-CD28刺激后,采用流式细胞术(FACS)检测IL-22的产生及其与IFN-γ、IL-17的关系,分析IL-22+T淋巴细胞CD45RO,CD62L,CCR7,CCR6,CCR10,CCR4等表面分子的表达。结果与anti-CD3+anti-CD28刺激外周血中CD4+和CD8+T淋巴细胞产生少量的IL-22(0.6%;0.57%)相比,肠道粘膜CD4+T细胞产生大约3.15%的IL-22,CD8+T淋巴细胞产生4%左右的IL-22。此外,肠道粘膜CD4+和CD8+T细胞中存在一群产生IL-22并独立于Th1、Th17,Tc1、Tc17的细胞亚群。肠道粘膜IL-22+T细胞表达较高比例的CD45RO,其中部分细胞表达CCR7,而较少表达CD62L。进一步研究表明,肠道粘膜CD4+IL-22+和CD8+IL-22+T细胞表达较高水平的CCR10(55.3%;73.9%),部分细胞表达CCR6或CCR4。结论人肠道正常粘膜组织中IL-22主要由效应型或中央型记忆T细胞产生,部分IL-22+T细胞独立于Th1、Th17,Tc1、Tc17细胞亚群。This paper aimed to compare the characteristics of IL-22-producing T cells in human normal membrane of intestinal tract and peripheral blood mononuclear cells（PBMC）.We isolated the mononuclear cells from the membrane of intestinal tract and peripheral blood,respectively.The production of IL-22 by T cells and the correlation of IL-22 with IFN-γ and IL-17 were detected by FACS after stimulation with anti-CD3 plus anti-CD28.Finally,the surface markers CD45RO,CD62L,CCR7,CCR6,CCR10 and CCR4 were analyzed.Compared with the lower secretion of IL-22 by CD4＋ and CD8＋ T cells from PBMCs（0.6%;0.57%）,3.15% of CD4＋ IL-22＋ and 4% CD8＋ IL-22＋ could be detected in membrane of intestinal tract after stimulation with anti-CD3 plus anti-CD28.A certain subset of CD4＋ IL-22＋ and CD8＋ IL-22＋ T cells was existed and they were distinct from Th1,Th17,Tc1,Tc17 cells.IL-22＋ T cells in the membrane of intestinal tract expressed higher levels of CD45RO,part of these cells expressed CCR7 and fewer expressed CD62L.Furthermore,CD4＋IL-22＋ and CD8＋IL-22＋ T cells expressed higher levels of CCR10（55.3%;73.9%） and part of CCR6 and CCR4 in the membrane of intestinal tract.In conclusion,IL-22 is mainly produced by the effector or central memory T cells from the membrane of intestinal tract.In addition,a certain subset of IL-22-producing T cells is distinct from Th1,Th17,Tc1,Tc17 cells.

关 键 词：IL-22 T淋巴细胞 表型特征 

分 类 号：R392.1[医药卫生—免疫学]