大剂量过敏原对致敏小鼠DC表面共刺激分子表达及调节性T细胞极化的作用  

Effects of high dose allergen on the expression of costimulatory molecules in DC and the amount of CD4^+CD25^+Foxp3^+ T cells in allergic mice

作　　者：宋云熙[1] 曾雪峰[2] 夏俊波 王长征[4] 张睢扬[1] 

机构地区：[1]第二炮兵总医院呼吸内科,北京100088 [2]四川省成都市第三人民医院呼吸科,610031 [3]浙江省杭州市第一医院呼吸科,310006 [4]第三军医大学新桥医院呼吸科,400037

出　　处：《免疫学杂志》2011年第10期879-882,共4页Immunological Journal

基　　金：国家自然科学基金资助项目(30640094)

摘　　要：目的观察和比较致敏小鼠及正常小鼠DC表面共刺激分子表达及CD4+CD25+Foxp3+T数量的差异及大剂量过敏原在体外的作用。方法流式细胞仪检测致敏及正常对照小鼠脾脏DC表面分子CD11c、MHCⅡ、CD80、CD86表达。分离致敏及正常对照小鼠CD4+T细胞,流式细胞仪检测CD4+CD25+Foxp3+T细胞的数量。致敏小鼠脾脏DC、CD4+T细胞与10 mg/ml OVA或生理盐水共培养后,流式细胞仪检测并比较CD80、CD86等共刺激分子的表达及CD4+CD25+Foxp3+T细胞的数量。结果致敏小鼠脾脏DC表面共刺激分子CD80、CD86、MHCⅡ表达显著高于正常对照小鼠。10 mg/ml的OVA作用后,致敏小鼠脾脏DC表面共刺激分子CD80、CD86的表达明显降低。致敏小鼠脾脏细胞中CD4+CD25+Foxp3+T细胞数量显著低于正常对照小鼠。10 mg/ml的OVA作用后,致敏小鼠CD4+CD25+Foxp3+T细胞数量显著上升。结论大剂量过敏原在体外诱导致敏小鼠T细胞的不反应性,其机制与降低致敏小鼠DC共刺激分子表达,诱导调节性T细胞极化等有关。We aimed to observe the effects of high dose allergen on the expression of costimulatory molecules and the amount of CD4＋CD25＋Foxp3＋ T cells.FACS was employed to detect the costimulatory molecules on spleen DC and the CD4＋CD25＋Foxp3＋ T cells in allergic mice and normal mice.It was found that the levels of MHCII,CD80 and CD86 were higher in allergic mice than those in normal mice,but the levels of CD80 and CD86 decreased when treated with OVA at 10 mg/ml.The amount of CD4＋CD25＋Foxp3＋ T cells separated from spleen was significantly decreased in allergic mice but it would increase when treated with OVA at 10 mg/ml.Thus we could conclude that T cell anergy can be induced by high dose allergen in allergic mice in vitro,and the underlying mechanism is related to the decrease of costimulatory molecules in DC and the increase of amount of CD4＋CD25＋Foxp3＋ T cells.

关 键 词：哮喘 树突状细胞 调节性T细胞 共刺激分子 

分 类 号：R392[医药卫生—免疫学]