小剂量瑞芬太尼对感染性休克幼兔体动脉张力的影响  

Effect of low-dose remifentanil on the systemic artery tension of baby rabbit with septic shock

作　　者：佟冬怡[1] 于泓波 李娜[3] 魏会霞[1] 张锦[1] 

机构地区：[1]中国医科大学附属盛京医院麻醉科,沈阳110004 [2]煤炭总医院麻醉科,北京100013 [3]沈阳市骨科医院麻醉科,110044

出　　处：《中国小儿急救医学》2011年第5期433-435,共3页Chinese Pediatric Emergency Medicine

基　　金：沈阳市科委重点课题（071021,101012）

摘　　要：目的观察小剂量瑞芬太尼（remifentanil，RF）对感染性休克幼兔体动脉血管环张力影响并探讨其机制。方法健康幼兔12只，分次制作36条幼兔离体体动脉血管环，随机分为6组：正常对照组、大肠杆菌脂多糖（1ipopoly—saccharide，LPS）对照组、正常＋RF组、LPS＋RF组、正常＋N-硝基左旋精氨酸（L—NNA）＋RF组和LPS＋L-NNA＋RF组，每组6个血管环。通过血管环张力测定，观察小剂量RF对感染性休克幼兔体动脉血管环反应性的影响，并观察预先以NO合成酶抑制剂L．NNA干预后的变化。结果正常组体动脉：与NS组比较，加入小剂量RF（4μg/L）后各时间点（5min、10min、20min）均引起体动脉明显的舒张反应，舒张幅度分别为（18．48±3．96）％、（23．63±4．42）％、（28．33±3．73）％（P〈0．05）。预先以L—NNA干预后，上述舒张反应明显降低至（8．15±1．01）％、（13．08±1．46）％、（18．54±2．94）％（P〈0．05）。LPS孵育后，小剂量RF（4μg／L）对幼兔体动脉不具舒张作用，与加入NS组比较差异无统计学意义（P〉0．05），预先以L—NNA干预后，体动脉对该剂量RF张力变化差异无统计学意义（P〉0．05）。结论小剂量RF对正常幼兔体动脉具有舒张作用，与NO产生有关；对LPS孵育的幼兔体动脉不具有舒张作用，对体动脉的张力调节与NO无关。Objective To investigate the effect and mechanism of low-dose remifentanil （RF） on systemic artery tension of baby rabbit with septic shock. Methods Thirty-six systemic artery strips were prepared from 12 baby rabbits and randomly divided into 6 groups： control group, lipopoly-saccharide （LPS） group, RF group, LPS ＋ RF group, NG-nitro-L-arginine （L-NNA） pretreated group, LPS ＋ L-NNA pretreated group. Each group had six rings （ n = 6）. The effect of low-dose RF on the systemic artery tension of baby rabbit with septic shock was observed with isolated vascular ring technique. The change was also obversed af- ter L-NNA pretreatment. Results In control group：the relaxant rates after low-dose RF at the time point of 5min,10min,20minwere （18.48 ±3.96）%,（23.63±4.42） %,（28.33±3.73）% （P〈0.05）,compared with NS group. After pretreatment with L-NNA, RF-related relaxant rates of systemic arteries decreased significantly to （8.15 ±1.01）%,（13.08 ±1.46）%,（18.54±2.94）% （P〈0.05）.In LPS group,low dose RF （4 μg/L）did not bring out any response to systemic arteries（ P 〉 0. 05 ）. The tension was not affected with pretreatment of L-NNA （ P 〉 0. 05 ）. Conclusion These results suggest that low dose RF has relaxant effect on systemic arteries of baby rabbits, and NO may be involved. Low dose RF has no relaxant effect on LPS-pretreated systemic arteries of baby rabbits, and it has no association with NO.

关 键 词：瑞芬太尼 感染性休克 体动脉 一氧化氮抑制剂 幼兔 

分 类 号：R965[医药卫生—药理学]