ITS序列在天门冬属物种DNA条形码构建中的应用研究  

Application research of ITS sequences DNA barcoding in asparagus species

作　　者：蒋向辉[1,2] 曾桂萍[3] 欧立军[1,2] 佘朝文[1,2] 许栋[1] 

机构地区：[1]怀化学院生命科学系,湖南怀化418008 [2]怀化学院民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418008 [3]贵州大学农学院,贵阳550025

出　　处：《华中师范大学学报（自然科学版）》2011年第3期472-476,共5页Journal of Central China Normal University：Natural Sciences

基　　金：贵州省中药现代化研究开发专项项目(黔科合农字[2006]5029号);湖南省科研计划重点资助项目(2009FJ2008);湖南省教育厅资助项目(09C755);湖南省教育厅重点实验室平台项目

摘　　要：在对天门冬属4个种ITS(internal transcribed spacer)序列克隆测序的基础上,并对4个种进行序列比较分析.结果表明:(1)4个物种的亲缘关系很近,它们间的ITS序列的变异小,GC含量高,在60%左右.(2)4个物种在系统树上聚为一类,文竹与其他3种天门冬的亲缘关系相对较远,处于系统树的一支,其中,非洲天门冬与新疆天门冬的亲缘关系较近,处于系统树的一支.(3)ITS序列的种间差异值略有不相,短梗天门冬与非洲天门冬的差异值为2.8%,与新疆天门冬的差异值为3.5%,与文竹的差异值为8.5%,非洲天门冬与新疆天门冬的差异值为2.1%,与文竹的差异值为8.3%,新疆天门冬与文竹的差异值为9.1%.综合分析认为:以ITS序列为DNA条形码可真实反应天门冬属物种间差异.This study made cluster analysis among the 4 spices of Asparagus, based on ITS（internal transcribed spacer） cloning sequencing. Firstly the result showed that there has close relationship and little variation of ITS sequence among 4 spices, with the highly 60% of GC content. Secondly, four spices belong to one kind. while Asparagus setaceus, in one branch of pylogenentic tree, has far genetic relationship with the other 3 Asparagus Species. And thirdly the result was in line with lianghu qu etal got . Once he got the interspesfic difference value of most family and genus ITS sequence of angiosperm varies from 1.2% to 10.2% via comparison with different live groups,and intergeneric difference value fluctuates from 9.6% to 28.8 %. The interspecific difference value between Asparagus lycopodineus and Asparagus densiflorus, Asparagus neglectus, Asparagus setaceus is as follows： 2.8%, 3.5%,8.5%. The difference value between Asparagus densiflorus and Asparagus neglectus, Asparagus setaceus is as follows ： 2.1% and 8.3%. The difference value between Asparagus neglectus and Asparagus setaceus is 9.1%. Our analysis suggests ITS sequences DNA barcoding can indicated real difference in Asparagus species.

关 键 词：ITS序列 天门冬属 DNA条形码 

分 类 号：Q819[生物学—生物工程]