1株产聚-β-羟基丁酸芽胞杆菌的分离、筛选与鉴定  

Isolation,Screening and Identification of A Poly-β-Hydroxybutyrate Yielding Bacillus Strain

作　　者：董夏梦[1] 宋迤明[1] 蒋冬花[1] 蓝丽精 蔡琪敏[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《微生物学杂志》2011年第4期80-84,共5页Journal of Microbiology

基　　金：浙江省公益性应用研究计划项目(2010C32076);浙江省大学生科技创新活动计划资助项目(2010R404064)

摘　　要：从浙江省金华市花生地土样中筛选、分离纯化得到1株产聚-β-羟基丁酸编号为PX-95的芽胞杆菌,PHB产量为135.81 mg/L。根据形态特征、生理生化特征初步鉴定为芽胞杆菌属蜡样芽胞杆菌群,16S rDNA序列分析显示PX-95菌株与苏云金芽胞杆菌以及蜡样芽胞杆菌均具有高度同源性,最后采用扩增gyrB(DNA促旋酶B亚单位)基因的方法将其鉴定为苏云金芽胞杆菌(Bacillus thuringiensis)。A Bacillus strain PX-95 producing poly-β-hydroxybutyrate（PHB） was isolated and purified from a peanut field soil,Jinhua,Zhejiang Province yielding PHB at 135.81 mg/L.According to its morphological and physiological features,it was preliminarily identified as a member of Bacillus cereus group.Analysis of its 16S rDNA sequence showed strain PX-95 had a highly homology to both Bacillus thuringiensis and Bacillus cereus.Finally it was characterized as Bacillus thuringiensis adopting amplification method of gyr B（DNA gyrase B subunit） gene.

关 键 词：聚-Β-羟基丁酸 苏云金芽胞杆菌 筛选 鉴定 

分 类 号：Q93-331[生物学—微生物学]