反相高效液相色谱法测定西荞3号黄酮含量  

Determination of Flavonoids in Tartary Buckwheat No.3 with RP-HPLC

作　　者：李艳[1] 谭涛[1] 张建红[1] 衡婷[1] 李沁媛[1] 刘思曼[1] 

机构地区：[1]绵阳师范学院化学与化学工程学院,四川绵阳621000

出　　处：《绵阳师范学院学报》2011年第8期52-54,66,共4页Journal of Mianyang Teachers＇ College

基　　金：绵阳市科技局自然科学研究项目(10S002-2);绵阳师范学院科学研究项目(hx2011-2012-06)

摘　　要：利用反相高效液相色谱法测定西乔3号中芦丁、槲皮素中成分的含量。采用SHIMDZU VP-ODS(4.6 mm×150 mm,5.0μm)色谱柱,甲醇-0.08%:磷酸溶液(6∶4)为流动相,流量为0.95 mL.min-1,检测波长为358 nm。待测组分与其他组分分离度良好(R>1.5),芦丁和槲皮素线性范围分别为32.0～288.0μg.ml-1和18.8～169.26μg.ml-1,平均回收率分别为98.5%,99.0%,相对平均偏差(n=5)分别为0.4%,0.4%。This paper is to introduce the experiment of determining the contents of rutin and quercetin in Tartary Buckwheat No.3 with RP-HPLC.Taking SHIMDZU VP-ODS column（4.6 mm×150 mm,5.0 μm）,a mixture of methanol-0.08% phosphoric acid in the ratio of 6 to 4 by volume with a flow-rate of 0.95 mL·min-1 and wavelength of 358 nm as the mobile phase,the range of linearity of rutin and quercetin（values of peak area and mass concentration）,after being well-separated,is between 32~288 μg·ml-1 and 18.2~169.2 μg·ml-1 respectively,the average rate of recovery for rutin is 98.5%,and 99.0% for quercetin with values of RSD＇s（n = 5） 0.4% and 0.4% respetively.

关 键 词：反相高效液相色谱 黄酮 西乔3号 

分 类 号：O657.72[理学—分析化学]