不同产地五鹤续断的同工酶研究  

Studies on Isozymes of Dipsacus from Different Origins

作　　者：何娜娜[1] 李慧[1] 王东辉[1] 匡华琴[1] 吴敏[1] 陈冬冬[1] 

机构地区：[1]湖北民族学院生物科学与技术学院,湖北恩施445000

出　　处：《湖北民族学院学报（自然科学版）》2011年第3期291-294,298,共5页Journal of Hubei Minzu University(Natural Science Edition)

基　　金：湖北民族学院园艺专业大学生创新项目;国家民委科研资助项目(08HB06)

摘　　要：为了分析恩施州不同产地续断之间的亲缘关系,采用聚丙烯酰胺凝胶垂直板电泳,对恩施州8个地区的五鹤续断进行了过氧化物酶和超氧化物歧化酶同工酶酶谱分析.8个地区的续断共电泳出5条POD同工酶酶谱和4条SOD同工酶酶谱,通过聚类分析得出,利川市元堡、恩施市双河地区的续断亲缘关系很近,鹤峰县太平乡和恩施市鱼塘坝上坝地区的续断亲缘关系也较近,而巴东县绿葱坡与其他地区的差异较大.In order to analyze genetic relation-ship Dipsacus from different origins in Enshi,Dipsacus from eight areas of Enshi were conducted on peroxidase and superoxide dismutase isozyme analysis,by using vertical polyacrylamide gel electrophoresis.A total of 5 POD and 4 SOD isozymes were electrophoresed out.The cluster analysis shhws that the Dipsacus in Yuanbao of Lichuan city and the Disacus in Shuanghe of Enshi City have very close genetic relationship,the Disacus in Taiping of Hefeng County and the Disacus in Yutangba of Enshi City also have close genetic relationship,but the Dipsacus in Lücongpo of Badong County is quite different from that of other areas.

关 键 词：续断 凝胶电泳 过氧化物酶 超氧化物岐化酶 

分 类 号：Q946.5[生物学—植物学] Q949.781.5