不同培养基对人牙周膜细胞生物学行为的影响  

Effects of different culture media on the biological behavior of human periodontal ligament cells

作　　者：唐焜琪[1] 钟泉[1] 李大兰[1] 姚丽艳[1] 李艳芬[1] 闫福华[1] 

机构地区：[1]福建医科大学口腔医学院.福建省高校口腔医学重点实验室,福建福州350002

出　　处：《口腔生物医学》2011年第3期120-125,共6页Oral Biomedicine

基　　金：高等学校博士学科点专项科研基金(20093518110002);福建医科大学苗圃科研基金(2010MP016);福建省科技重点项目(2009Y0019)

摘　　要：目的:研究α-MEM、DMEM-LG、RPMI1640三种培养基对人牙周膜细胞(human periodontal ligament cells,hPDLCs)生物学行为的影响,为体外培养人牙周膜细胞寻找合适的培养基。方法:采用改良组织块培养法,分别用α-MEM、DMEM-LG、RPMI1640三种培养基对hPDLCs进行体外培养,比较三组细胞在游出成功率、增殖及矿化等方面的差异。结果:α-MEM促进hPDLCs增殖作用最强。α-MEM组培养的细胞表达的碱性磷酸酶(alkaline phosphatase,ALP)含量最高,与其它两组间有显著差异。DMEM-LG组矿化结节形成个数最多且体积大。结论:α-MEM促进hPDLCs增殖,DMEM-LG促进hPDLCs分化。应根据实验要求选择合适培养基。Objective：To observe the effects of three different culture media： α-MEM ,DMEM-LG and RPMI 1640 on the t）ioh）gical behavior of human periodontal ligament cells （hPDLCs） , and provide the best culture medium for cultured hPDLCs in vitro. Methods： hPDLCs were cuhured in α-MEM ,DMEM-LG and RPMI 1640 respectively in vitro with the improved tissue block culture method. The successful cell migration rate, proliferation and mineralized nodules of cells were observed and compared. Results ： The proliferation of cells cultured by ot-MEM was significantly increased. In addition, α-MEM strongly induced alkaline phosphatase activity. But DMEM- LG group exhibited the most mineralized nodules with biggest size. Conclusions：α-MEM is suitable for proliferation of hPDLCs in vitro. DMEM-LG can, promote the differentiation of hPDLCs. It could be ascertained to choose appropriate culture medium according to the requirement of different experiments.

关 键 词：人牙周膜细胞 培养基 生物学活性 

分 类 号：Q813.11[生物学—生物工程]