SD大鼠下颌第一磨牙DLX5、RUNX2、OSX、DSP的表达分析  

Study on the expression of DLX5,RUNX2,OSX, DSP in mandibular first molar of postnatal SD rat

作　　者：俞艳[1,2] 曹灵[1,3] 闫明[1,2] 于金华[1,2] 张光东[1,2] 

机构地区：[1]南京医科大学口腔医学研究所,江苏南京210029 [2]南京医科大学附属口腔医院牙体牙髓病科,江苏南京210029 [3]南京医科大学附属口腔医院儿童牙病科,江苏南京210029

出　　处：《口腔生物医学》2011年第3期126-130,共5页Oral Biomedicine

摘　　要：目的:观察远端缺失同源盒5(distal-less homeobox5,DLX5)、核心结合蛋白因子2(runt-related transcription factor-2,RUNX2)、成骨细胞特异性转录因子Osterix(OSX)和牙本质涎蛋白(dentin sialoprotein,DSP)在出生后SD大鼠下颌第一磨牙不同发育阶段的表达情况,探讨其在SD大鼠出生后下颌第一磨牙发育矿化的作用机制。方法:采用免疫组化法分析出生后1、7、14、28d SD大鼠下颌第一磨牙中DLX5、RUNX2、OSX、DSP的表达分布。结果:DLX5在出生后1、7、14d中均有表达,表达量呈现出上升趋势,但是在28d时不表达;RUNX2在出生后1、7、14、28d均表达,表达量在7d时呈现最低;OSX在出生后1、7、14、28d均有表达,表达量在14d时呈现最低;DSP在出生后1、7、14、28d均有表达,表达量呈上升趋势。结论:DLX5、RUNX2、OSX、DSP在大鼠出生后各阶段的表达量不同,具有时空特异性,表明DLX5、RUNX2、OSX、DSP可能对出生后SD大鼠下颌第一磨牙发育及矿化具有一定的调控作用。Objective： To observe the expression of distall-less homeobox 5 （ DLX5 ）, runt-related transcription factor 2 （ RUNX2 ）, Osterix （OSX） and dentin sialoprotein （DSP） in mandibular first molars of postnatal （PN） SD rat. Methods： SD rat mandibles were isolated and fixed in 4% polyoxymethylene at PN 1, 7, 14, 28 days respectively. Tissue sections from paraffin blocks were processed for HE and immunohistochemistry staining to detect the expression of DLXS, RUNX2, OSX and DSP. Results ：The expression of DLX5 was detected in specimen at PN 1,7, 14 days and displayed an uprising positive staining, but negative at PN 28 days. RUNX2 was de- tected in specimen at PN 1, 7, 14 and 28 days, among which the expression level was the lowest at PN 7 days. OSX was detected in specimen at PN 1, 7, 14 and 28 days, among which the expression level was the lowest at PN 14 days. An uprising trend of OSX levels was detected in specimen at PN 1,7, 14 and 28 days. Conclusions ： The expression of DLXS, RUNX2, OSX and DSP in postnatal SD rat mandibular first molar was time-specific, reflecting their different functional state following the odontogenesis.

关 键 词：远端缺失同源盒5 核心结合蛋白因子2 OSTERIX 牙本质涎蛋白 下颌第一磨牙 大鼠 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]