鹅掌楸属植物组织培养研究进展  

Advances in tissue culture of Liriodendron

作　　者：刘静[1] 

机构地区：[1]安康学院,陕西安康725000

出　　处：《南方农业学报》2011年第9期1045-1048,共4页Journal of Southern Agriculture

基　　金：陕西省自然科学基础研究计划项目(2009JM3017)

摘　　要：近年来,我国对鹅掌楸属植物种苗的需求不断增加,但由于鹅掌楸属植物花期不遇,种子发芽率低,扦插、嫁接繁殖比较困难,因此组织培养技术成为鹅掌楸属植物种苗快速繁殖的一条重要途径。文章从外植体的选择、初代和继代培养、培养条件、生根培养及体细胞胚胎发生等方面综述了鹅掌楸属植物组织培养的研究成果,分析鹅掌楸属植物组织培养中存在的问题,探讨鹅掌楸属植物组织培养研究的方向。今后将继续研究解决鹅掌楸属植物组培过程中生根难、褐化等问题,加强在原生质体水平上对鹅掌楸属植物再生繁殖体系及其遗传转化体系的研究;研究利用组织培养技术对鹅掌楸属植物进行种质保存,从而为其种质资源的保存和利用、新品种的选育提供技术支持。In recent years, the demand of Liriodendron seedlings in China is enhanced significantly. Due to asynchronized flowering, lower germination rate of seeds, and difficult cutting-grafting propagation, the rapid propagation using tissue culture techniques became an important approach to produce plantlets of Liriodendron. The research progresses in tissue culture techniques used for Liriodendro plants, e.g., screening explants, primary culture and subculture, culture medium, hormone, culture conditions and somatic embryogenesis were summarized in the current paper. The existing problems and countermeasures also were discussed in the paper. The emphasis has been given on future studies involving the techniques to overcome difficulties in rooting and browning during the process of tissue culture in Liriodendron, and development of efficient protoplast culture and genetic transformation system. The germplasm protection of Liriodendron plants using tissue culture technology should be studied to provide technological support for germplasm resource utilization and help in breeding of new varieties.

关 键 词：鹅掌楸属 组织培养 研究进展 展望 

分 类 号：S792.21[农业科学—林木遗传育种]