陶瓷复合膜分离工艺生产新生牛血清的研究  

Production of ceramic composite membrane separation process of newborn calf serum

作　　者：庞观龙[1] 周贞兵[1] 

机构地区：[1]广西农业职业技术学院,南宁530007

出　　处：《南方农业学报》2011年第9期1157-1160,共4页Journal of Southern Agriculture

基　　金：广西科学基金和应用基础研究专项项目(桂科基0832034)

摘　　要：【目的】探索一次性过滤截留新生牛血清中细菌、霉菌、支原体等微生物及免疫球蛋白的工艺技术,为新生牛血清规模生产提供技术支撑。【方法】采用陶瓷复合膜分离生产新生牛血清,经细菌、支原体、牛病毒性腹泻病毒(BVDV)抗体、细菌内毒素等微生物及免疫球蛋白检测,并进行新生牛血清产品细胞培养试验。【结果】陶瓷复合膜能一次性成功截留新生牛血清中的细菌、支原体、细菌内毒素、BVDV抗体,过滤后细菌内毒素含量低于0.1EU/mL,免疫球蛋白去除率在97.00%以上;以其培养SP2/0细胞的细胞倍增时间、单克隆效率等指标接近于进口Hyclone胎血牛清水平。【结论】采用陶瓷复合膜分离工艺生产新生牛血清,实现了一次性有效过滤截留牛血清中细菌、霉菌、支原体等微生物及免疫球蛋白的目标,且能够满足细胞生长的营养需求,为大批量生产优质新生牛血清产品奠定了基础。【Objective】The present study was conducted to develop the techniques for disposable filtration and retention of bacteria, fungi, mycoplasma, etc., in newborn calf serum and the immunoglobulin to provide the support on production of newborn calf serum on large scale. 【Methods】The ceramic composite membrane was used to produce the newborn calf serum, and the bacteria, mycoplasma, bovine viral diarrhea virus （BVDV） antibodies, bacterial endotoxin and other microbial and immunoglobulin in serum were detected. The newborn calf serum products were used for the cell culture experiment. 【Results】The ceramic composite membrane successfully retained the bacteria, mycoplasma, bacterial endotoxin and BVDV antibodies in serum. After filtration, the bacterial endotoxin content in fetal calf serum was less than 0.1 Eu/mL, and the removal rate of immunoglobulin was over 97.00%. The doubling time of cell, monoclonal efficiency of SP2/0 cells in filter serum medium had no difference to that of import Hyclone fetal serum. 【Conclusion】Applying ceramic composite membrane separation process to produce newborn calf serum could be effectively achieved by disposable filtration and retention of bacteria, fungi, mycoplasma and the immunoglobulin in bovine serum, and could meet the nutritional need in growth of cells, which will provide basis on mass production of newborn calf serum high-quality product.

关 键 词：新生牛血清 陶瓷复合膜 分离 免疫球蛋白 

分 类 号：S852.5[农业科学—基础兽医学]