水稻种子相关细菌的系统发育分析与促生能力评价  

Analysis on growth-promoting ability and phylogeny with rice seed associated bacteria

作　　者：曹艳花[1] 徐凤花[1] 陈小忠[3] 张晓霞[2] 韦善君[3] 马晓彤[2] 

机构地区：[1]东北农业大学资源与环境学院,黑龙江哈尔滨150030 [2]中国农业科学院农业资源与农业区划研究所,中国农业微生物菌种保藏管理中心,北京100081 [3]中央民族大学生命与环境科学学院,北京100081

出　　处：《中国土壤与肥料》2011年第5期83-87,共5页Soil and Fertilizer Sciences in China

基　　金：国家自然科学基金项目(No.30900001);中央级公益性科研院所基本科研业务费专项(No.2011-11)

摘　　要：对15株分离自水稻冀粳14号(90-3)的种子表面及其内生细菌进行了多功能筛选评价和16S rRNA系统发育分析。结果表明,供试菌株都能够溶解无机磷,不能分解有机磷;筛选获得6株具有明显分泌植物生长素吲哚-3-乙酸(IAA)能力的菌株;12株菌株明显产生铁载体,占所测菌株总数的80%,其中7株铁载体产量达到中等水平,通过16S rRNA基因系统发育及聚类分析,供试菌株聚为8个类群,分别属于Rhizobium、Citrobacter、Xanthomonas、Psedomonas、Bacillus、Pantoea、Microbacterium和Moraxella属。In this paper,15 seed associated strains isolated from rice（Oryza sativa L.subsp.japonica 90-3）seeds were studied by means of phylogenetic analysis of 16S rRNA gene and detection the abilities of phosphate solubilization,Indole-3-Acetic Acid（IAA）secretion and siderophores biosynthesis.The main results were as follows： all the tested strains were able to dissolved inorganic phosphorus,and insoluble organic P;Six strains could secrete IAA obviously;The siderophore production of seven strains reached moderate level.The tested strains were be longing to the genus of Rhizobium,Citrobacter,Xanthomonas,Psedomonas,Bacillus,Pantoea,Microbacterium and Moraxella according to 16S rRNA gene phylogenetic analysis.

关 键 词：水稻种子相关细菌 系统发育 溶磷 IAA分泌 铁载体合成 

分 类 号：S511[农业科学—作物学]