IL-2对COPD患者CD8^+ T细胞频率及功能的影响  

Effect of IL-2 on frequency and function of CD8^+ T cells in COPD patients

作　　者：徐晟伟[1] 沈若武[2] 张蓓[2] 

机构地区：[1]青岛市第三人民医院,山东青岛266021 [2]青岛大学医学院

出　　处：《山东医药》2011年第33期22-24,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81072398);山东省科技发展计划项目(2009GG10002044)

摘　　要：目的探讨IL-2在慢性阻塞性肺疾病(COPD)中的免疫调节作用。方法采集45例COPD急性发作期患者、33例COPD缓解期患者以及25例健康人外周血,采用ELISA检测血清中IFN-γI、L-2浓度。提取PBMC后分别设置IL-2刺激组和未刺激组,多色流式分析技术检测CD8+T细胞频率,胞内细胞因子染色技术检测CD8+IFN-γ+T细胞的频率。结果 COPD患者急性发作期IL-2I、FN-γ浓度较临床缓解期和对照组显著增高(P均<0.01);与未刺激组相比I,L-2刺激组CD8+T细胞频率及分泌IFN-γ显著增加(P<0.05)。结论 IL-2可通过对CD8+T细胞的免疫调节效应参与COPD的发生发展过程。Objective To investigate the immunoregulatory role of IL-2 in the chronic obstructive pulmonary disease（COPD）.Methods IL-2 and IFN-γ in serum from 78 patients with COPD and 25 healthy subjects were quantitively analyzed using enzyme-linked immunosorbent assay（ELISA）.The frequency of CD8＋ T cells was analyzed by flow cytometry.IFN-γ was analyzed using intracellular cytokine straining.Results The serum concentrations of IL-2 and IFN-γ in acute exacerbations of COPD patients were significantly higher than those in stable and control groups（all P〈0.01）.There were higher frequencies of circulating CD8＋T cells and CD8＋IFN-γ＋T cells in the IL-2 stimulation group than those of no IL-2 stimulation group（all P〈0.05）.Conclusons IL-2 play an important role in the genesis and development of COPD by immunoregulating the frequency and function of CD8＋ T cells.

关 键 词：IL-2 肺疾病 慢性 阻塞性 CD8+T细胞 

分 类 号：R563[医药卫生—呼吸系统]