马铃薯脱毒试管苗保苗与试管薯诱导条件优化  

Studies on maintaining virus-free potato plantlets and optimization of microtuber induction

作　　者：刘艳芬[1] 陈翠果[1] 梁伟玲[1] 暴建枝[1] 胡爱双[1] 李全红[1] 

机构地区：[1]河北工程大学农学院,河北邯郸056002

出　　处：《浙江农业学报》2011年第5期876-879,共4页Acta Agriculturae Zhejiangensis

摘　　要：研究了不同外源激素组合对马铃薯脱毒试管苗保苗效果的影响,研究了培养基、温度、光周期的综合作用对试管薯发生的影响。试验表明,适合于试管苗保苗的液体培养基为MS+CCC 4.0 mg.L-1+6-BA 0.5mg.L-1+GA30.05 mg.L-1,适宜试管薯诱导的培养基和培养条件为:液体培养基MS+6-BA6.0 mg.L-1+白糖60 g.L-1+活性炭3 g.L-1,环境温度18℃,光照10 h.d-1。The effects of exogenous hormone on maintaining of virus-free potato plantlets and the combined effects of medium, temperature and photoperiod on microtuber induction were studied. The results showed that the optimal medium of maintaining plantlets was MS ＋ CCC 4. 0 mg· L^-1 ＋ 6-BA 0. 5 mg. L^-1 ＋ GA3 0. 05 mg· L^-1 , the optimal media to induce mierotubers was MS ＋ 6-BA 6. 0 mg·L^-1 ＋ sugar 60 g· L ^-1 ＋ active carbon 3 g·L^-1, temperature at 18℃, and brightness for 10 h·d^-1.

关 键 词：马铃薯试管苗 保苗 试管薯 优化 

分 类 号：S451[农业科学—植物保护]