浙贝母鳞茎愈伤组织诱导分化及植株再生研究  

Callus induction,differentiation and plant regeneration of bulbs of different cultivars of Fritillaria thunbergii

作　　者：俞超[1] 沈晓霞[2] 王忠华[1] 傅佳丽[1] 郑霏[1] 

机构地区：[1]浙江万里学院生物技术研究所,浙江宁波315100 [2]浙江省中药研究所,浙江杭州310023

出　　处：《浙江农业学报》2011年第5期910-913,共4页Acta Agriculturae Zhejiangensis

基　　金：浙江省科技厅重点项目(2009C12088)

摘　　要：以狭叶、宽叶和多籽浙贝母品种鳞茎为材料,研究了不同消毒方法、不同激素配比培养基对浙贝母愈伤组织诱导和分化及植株再生的影响。结果表明,0.1%升汞处理20 min为最佳的消毒方法;在诱导培养基MS+NAA1.5 mg.L-1+KT0.5 mg.L-1中诱导率最高,达到36.7%;在分化培养基MS+NAA 4.0 mg.L-1+KT1.0 mg.L-1中分化率最高,达到80.7%。研究结果还发现,不同基因型浙贝母对鳞茎愈伤组织诱导与分化及植株再生有较大影响。The bulbs of narrow-leaf, broad-leaf and multi-seed cultivars of Fritillaria thunbergii Miq were used to investigate the effect of different sterilized methods and culture media with different hormone combinations on the callus induction, differentiation and plantlets regeneration. The results showed that the optimum sterilized method was treated with 0. 1% mercuric chloride for 20 rain ; the callus induction rate was the highest on the medium with MS ＋ NAA 1.5 mg -L^-1 ＋ KT 0. 5 mg .L^-1, and the induction rate was 36. 7% ; the callus differentiation rate was the highest on the medium with MS ＋NAA 4. 0 mg .L^-1 ＋KT I. 0 mg .L^-1, and the differentiation rate was 80. 7%. The results also demonstrated that the genotype had significant effects on callus induction, differentiation and plant regeneration of different cultivars of Fritillaria thunbergii.

关 键 词：浙贝母 鳞茎 愈伤组织 诱导 分化 植株再生 

分 类 号：S567.231[农业科学—中草药栽培]