好氧反硝化细菌的筛选鉴定及其反硝化反应条件优化  

Screening and identification of aerobic denitrifiers and the optimization of denitrification conditions

作　　者：周莉[1,2] 汤江武[2] 王新[2] 姚晓红[2] 吴逸飞[2] 葛向阳[1] 

机构地区：[1]华中农业大学生命科学与技术学院,湖北武汉430070 [2]浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

出　　处：《浙江农业学报》2011年第5期942-947,共6页Acta Agriculturae Zhejiangensis

摘　　要：从养殖水体、污泥和农村河道中定向筛选好氧反硝化细菌,对分离得到的菌株进行初步鉴定,并研究了不同碳源、碳氮比、初始pH、接种量、转数以及温度等对其反硝化特性的影响。结果表明,从初筛得到的35株具有反硝化活性的细菌中复筛得到一株具有较强反硝化能力的菌株GC5,经16S rDNA测序和系统发育分析,该菌株属于假单胞菌属(Pseudom onassp.)。在以乙醇为碳源、碳氮比15∶1,接种量1%,初始pH 7.5,转数160 r.m in-1和温度30℃的条件下,脱氮效率最强,对模拟污水中硝酸盐氮和总氮的去除率分别为99.19%和53.83%。Aerobic denitrifying bacteria were isolated directly from the aquaculture water, sludge and rural rivers. Following characterizing the phylogeny of the strains with the highest denitrifying capability, the effects of carbon source, C/N ratio, initial pH, inoculation quantity, rotational speed and temperature on the characteristics of denitrification were studied. The results showed that 35 strains of aerobic denitrifiers were obtained by primary screening on plates of Bromothymol Blue medium. One strain named GC5, which had the highest denitrifying capability was identified after secondary screening and was designated as Pseudomonas sp. based on the analysis of 16S rDNA sequence and phylogenetic analysis. Furthermore, the optimum conditions for denitrification were as follows： carbon sources ethanol, C/N ratio 15： 1, inoculation quantity 1% , initial pH 7.5 ,rotational speed 160 r-min^-1, and temperature 30℃ . Under such condition, the removal rate of nitrate nitrogen and total nitrogen in simulation sewage were 99. 19% and 53.83%, respectively.

关 键 词：好氧反硝化 定向筛选 反硝化特性 假单胞菌属 

分 类 号：Q939.112[生物学—微生物学]