缬草提取物8-羟基松脂醇苷对Kv1.5钾通道电流的影响  

Effects of 8-hydroxy Resin Glycosides from Valeriana officinalis L.on Kv1.5 Potassium Channel Currents

作　　者：方颖[1] 段雪云[1,2] 王宏飞[3] 范恒[3] 刘焱文[1] 

机构地区：[1]湖北中医药大学湖北省中药资源与中药复方省部共建教育部重点实验室,武汉430065 [2]湖北省中医院药学部,武汉430065 [3]华中科技大学同济医学院附属协和医院中西医结合科,武汉430022

出　　处：《医药导报》2011年第10期1259-1262,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(基金编号:30500053)

摘　　要：目的探讨缬草提取物8-羟基松脂醇苷对Kv1.5钾通道电流的影响。方法以转基因Kv1.5HEK293细胞为受试对象,采用膜片钳技术观察10,30μmol.L-18-羟基松脂醇苷对转基因Kv1.5HEK293细胞电流的影响。结果 8-羟基松脂醇苷浓度依赖性减少Kv1.5HEK293细胞电流,电流值从(148.3±13.0)PA/PF分别降至(109.4±4.4)PA/PF和(78.6±11.3)PA/PF,抑制率分别为28.5%和37.1%。结论 8-羟基松脂醇苷对转基因Kv1.5 HEK293细胞电流有抑制作用,且呈浓度依赖性,可以部分解释缬草抗心律失常作用机制。Objective To study effects of 8-hydroxy resin glycosides from Valeriana officinalis L.on Kv1.5 potassium channel currents. Methods The Kv1.5 HEK293 cells currents following the administration of 8-hydroxy resin glycosides at 10 and 30 μmol·L-1 were recorded by using the whole-cell patch clamp recording technique. Results 8-hydroxy resin glycosides（10 and 30 μmol·L-1） decreased Kv1.5 HEK293 cells currents in a dose-dependent manner.The peak currents（148.3±13.0） PA/PF was reduced to（109.4±4.4） PA/PF and（78.6±11.3） PA/PF,which resulted in the suppression ratio of 28.5% and 37.1%,respectively. Conclusion 8-hydroxy resin glycosides blocks Kv1.5 HEK293 cells currents dose dependently,which may contribute to the anti-arrhythmic effects of Valeriana officinalis L..

关 键 词：8-羟基松脂醇苷 缬草 Kv1.5HEK293细胞 Kv1.5钾通道 

分 类 号：R285.6[医药卫生—中药学]