高效液相色谱法测定酰嘧磺隆、乙氧磺隆、烟嘧磺隆原药的含量  

Determination of Amidosulfuron,Ethoxysulfuron and Nicosulfuron TC by HPLC

作　　者：范志先[1] 张晖[1] 李娇[1] 

机构地区：[1]青岛科技大学化工学院,山东青岛266042

出　　处：《现代农药》2011年第5期34-37,共4页MODERN AGROCHEMICALS

摘　　要：对以20 mmol/L甲酸+10 mmol/L甲酸铵作为调节剂,采用乙腈/水为流动相,C8柱分离,PDA检测器测定酰嘧磺隆、乙氧磺隆、烟嘧磺隆原药的高效液相色谱分析方法进行了研究。酰嘧磺隆以苯甲酸甲酯为内标,乙腈比例40%,230 nm检测;乙氧磺隆以邻苯二甲酸二甲酯为内标,乙腈比例55%,222 nm检测;烟嘧磺隆以邻苯二甲酸二甲酯为内标,乙腈比例30%,232 nm检测。在25～500 mg/L范围内,线性方程分别为Y=1.1042 X+0.0152,Y=0.7606 X+0.0064,Y=1.122 4 X+0.005 6。r值分别为0.999 9,0.999 8,0.999 2。添加回收率分别为99.37%～99.43%,99.09%～99.38%,99.79%～99.84%。RSD分别为0.85%,0.46%,0.27%。建立的3种嘧啶磺酰脲类除草剂原药的内标定量分析方法可将原药及杂质/降解产物同时分离,定量结果准确可靠。A method for analysis of amidosulfuron,ethoxysulfuron and nicosulfuron TC by HPLC was developed.Samples were dissolved in acetonitrile,with acetonitrile / water as mobile phase,10 mmol/L ammonium formate and 20 mmol/L formic acid as regulator.C8 column and photo diode array detector were used for quantitative determination.Amidosulfuron were detected at 230 nm,with methyl benzoate as internal standard and the ratio of acetonitrile in eluent was 40 %.Ethoxysulfuron was detected at 222 nm,with dimethyl phthalate as internal standard and the ratio of acetonitrile was 55 %.Nicosulfuron was detected at 232 nm,with dimethyl phthalate as internal standard and the ratio of acetonitrile was 30 %.In the range of 25-500 mg/L,the linear equations were Y=1.1042 X＋0.0152,Y=0.7606 X＋0.0064,Y=1.1224 X＋0.0056 and r were 0.9999,0.9998,0.9992,respectively.The recoveries were 99.37%-99.43%,99.09%-99.38%,99.79%-99.84% and RSD were 0.85%,0.46%,0.27%,respectively.The developed internal standard method can quantitatively determinate the three technical materials and separate the technical from impurities/degrada-tion products simultaneously.

关 键 词：酰嘧磺隆 乙氧磺隆 烟嘧磺隆 原药 高效液相色谱法 

分 类 号：S482.4[农业科学—农药学]