低强度脉冲电磁场对兔动脉粥样硬化斑块中MMP-9及TIMP-1表达的影响  

Effects of low-intensity pulse electromagnetic field on expression of MMP-9 and TIMP-1 in atherosclerotic plaques of rabbits

作　　者：李秀川[1,2] 陈易华[3] 马双陶[2] 杨大春[2] 罗艳[3] 邱力军[1] 

机构地区：[1]第四军医大学生物医学工程系,西安710032 [2]成都军区总医院心血管内科 [3]成都军区总医院病理科

出　　处：《西南国防医药》2011年第10期1064-1067,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的探索低强度脉冲电磁场对大白兔动脉粥样硬化(AS)斑块中基质金属蛋白酶-9(MMP-9)及组织金属蛋白酶抑制物-1(TIMP-1)表达的影响。方法将大白兔随机分成常规饮食(ND)组、高脂饮食(HD)组及高脂饮食+磁场(HM)组,ND及HD组分别给予常规饮食及高脂饮食,HM组动物在给予高脂饮食的同时,加用低强度脉冲电磁场进行干预。干预7 w后,取主动脉根部行常规石蜡切片及HE染色后,计算AS斑块的面积,采用免疫组化及蛋白免疫印迹法检测AS斑块中MMP-9和TIMP-1的表达。结果高脂饮食可显著增加主动脉根部AS斑块的面积(P<0.05),低强度脉冲电磁场干预能显著削弱高脂饮食诱导的AS斑块形成(P<0.05);高脂饮食能显著增加AS斑块中MMP-9和TIMP-1的表达(P<0.05),而低强度脉冲电磁场干预能显著抑制高脂饮食诱导的MMP-9和TIMP-1表达水平的增加(P<0.05),且可显著降低MMP-9与TIMP-1的比值。结论低强度脉冲电磁场抗AS的作用机制可能与降低MMP-9与TIMP-1的比值有关。Objective To investigate the effects of low-intensity pulse electromagnetic field（LPEF）on the expression of matrix metalloproteinase-9（MMP-9）and tissue inhibitor of metalloproteinase-1（TIMP-1）in atherosclerotic（AS）plaques of rabbits.Methods Rabbits were randomly divided into three groups：normal diet（ND）group,high fat diet（HD）group and HD plus magnetic field（HM）group.The ND and the HD group were respectively given regular diet and high fat diet.The HM group was given high fat diet,and meanwhile received intervention of LPEF.After intervention for 7 w,the aortic roots of these animals were dissected for routine paraffin sections and HE staining,and the area of AS plaque was calculated.In addition,the expression of MMP-9 and TIMP-1 in AS plaque was detected by immunohistochemical staining.Results HD treatment significantly increased the area of AS plaque in aortic root compared with ND treatment（P0.05）.LPEF intervention significantly attenuated HD-induced formation of AS plague（P0.05）.HD treatment significantly elevated the expression of MMP-9 and TIMP-1 in AS plaque（P0.05）.However,the intervention of LPEF significantly inhibited the HD-induced increases of the expression level of MMP-9 and TIMP-1（P0.05）,and remarkably decreased the ratio of MMP-9 to TIMP-1.Conclusion The anti-AS mechanism of LPEF treatment may be related to decrease the ratio of MMP-9 to TIMP-1.

关 键 词：动脉粥样硬化 低强度脉冲电磁场 MMP-9 TIMP-1 

分 类 号：R543.12[医药卫生—心血管疾病]