胶体金免疫层析快速定量检测西尼罗病毒抗体方法的建立  

Rapid quantitative detection on antibody of West Nile Virus using colloid gold immunochromatographic test

作　　者：杨宇[1] 谢士嘉[1] 王静[1] 秦成峰[2] 

机构地区：[1]中国检验检疫科学研究院卫生检疫研究所,北京100123 [2]军事医学科学院微生物流行病研究所

出　　处：《中国国境卫生检疫杂志》2011年第5期307-310,共4页Chinese Journal of Frontier Health and Quarantine

基　　金：质检公益项目(2007GYJ024;2007GYJ023);国家质检总局科技计划项目(2010IK211)

摘　　要：目的建立一种胶体金免疫层析快速定量检测西尼罗病毒抗体的方法。方法利用胶体金免疫层析技术,建立快速检测西尼罗病毒抗体的方法,评价其特异性和敏感性,进行定量检测并拟合标准曲线。结果该法可在15min内完成定性和定量检测,定量检测灵敏度为1:104/test,线性范围1:101/test～1:104/test、回收率86.5%～122.7%。结论建立的检测西尼罗病毒抗体的胶体金免疫层析方法,能快速、灵敏、特异、准确地对样本中的西尼罗病毒抗体进行定性、定量检测。Objective To develop a rapid and quantitative detection method on antibody of West Nile Virus using colloid gold immunochromatographic test. Methods The rapid detection method was established with colloid gold immunochromatographic assay, and the specificity and sensitivity of the method was then evaluated. Quantification was realized by constructing a standard curve. Results This established method could be accomplished qualitatively as well as semi-quantitative detection in 15 minutes, the sensitivity was up to 1：10^4/test, the linear range was froml：10^4/test to 1： 10^4/test, the recovery rate was 86.5%-122.7%. This method showed good specificity and sensitivity, which could detect samples directly. Conclusion The colloid gold immunochromatographic test we constructed could detect the antibody of West Nile Virus in samples rapidly, specifically, sensitively and accurately, and to realize quantification, and is suitable for field detection.

关 键 词：西尼罗病毒 胶体金 免疫层析 定量检测 

分 类 号：R512.3[医药卫生—内科学]