两种结核分枝杆菌快速检测方法的比较  

Comparision of two kinds of rapid test mothods for mycobacterium tuberculosis

作　　者：吴健[1] 张建明[1] 何洪涛[1] 陈建东[1] 黄永欣[1] 雷兆良[1] 朱云枫[1] 黄红敏[1] 

机构地区：[1]广东国际旅行卫生保健中心,广东广州510635

出　　处：《中国国境卫生检疫杂志》2011年第5期315-317,325,共4页Chinese Journal of Frontier Health and Quarantine

基　　金：广东出入境检验检疫局科技计划项目(GDK01-2000)

摘　　要：目的比较荧光定量PCR检测和直接扩增检测两种方法对结核分枝杆菌检测的敏感性、特异性,及在国境口岸卫生检疫工作中的应用前景。方法应用结核分枝杆菌荧光定量PCR和直接扩增检测47份疑似或确诊活动性肺结核空腹痰标本,并与液体培养结果进行比较。结果荧光定量PCR法与直接扩增法对结核分枝杆菌检测敏感性分别为46.7%和100%,特异性分别为81.2%和84.4%。结论结核分枝杆菌直接扩增检测是一种较为敏感而特异的快速检测方法,在国境口岸卫生检疫部门具有重要的应用价值。Objective To compare the sensitivity and specificity of two kinds of rapid test of mycobacterium tuberculosis （fluorescence quantitative PCR and Amplified MTB Direct）, and to evaluate their practical value at port. Methods The specimens of sputum were isolated from 47 cases of suspected or diagnosed tuberculosis patients, M.tuberculosis were detected by fluorescence quantitative PCR and Amplified MTB Direct respectively. The results were compared with that of the liquid culture. Results The sensitivity of fluorescence quantitative PCR and Amplified MTB Direct were 46.7% and 100% respectively, and the specificity were 81.2% and 84.4% respectively. Conclusion Amplified MTB Direct method is a rapid, sensitive and specific method for the diagnosis of tuberculosis. It has a practical value for TB control at port.

关 键 词：结核分枝杆菌 液体培养 荧光定量PCR 结核分枝杆菌直接扩增检测 

分 类 号：R183.3[医药卫生—流行病学]