2010年中蒙边境甘其毛都口岸地区蜱携带病原体调查研究  

Investigation on tick-borne pathogens at Ganqimaodu port between Sino-Mongolia border

作　　者：杨宇[1] 张晓龙[1] 乔舜 曹晓梅[1] 王林[1] 郝广福 胡健萍[1] 杨志红[1] 王静[1] 

机构地区：[1]中国检验检疫科学研究院卫生检疫研究所,北京100123 [2]内蒙古出入境检验检疫局

出　　处：《中国国境卫生检疫杂志》2011年第5期339-342,共4页Chinese Journal of Frontier Health and Quarantine

基　　金：自然科学基金(30900053);财政部质检公益项目(2007GYJ025)

摘　　要：目的为了解中蒙边境蜱传疾病病原体携带情况,对2010年中蒙联合监测小组在内蒙古乌拉特中旗甘其毛都口岸地区采集的162只亚东璃眼蜱进行病原体检测。方法采用RT-PCR法检测蜱标本中的森林脑炎病毒、克里米亚-刚果出血热病毒、布尼亚病毒,PCR方法检测蜱标本中的伯氏疏螺旋体、西伯利亚立克次体、贝氏柯克斯体,嗜吞噬细胞无形体。结果森林脑炎病毒、克里米亚-刚果出血热病毒、布尼亚病毒、嗜吞噬细胞无形体检测结果均为阴性,PCR方法检出5例伯氏疏螺旋体、3例西伯利亚立克次体、16例贝氏柯克斯体,检出率分别为3.7%、2.2%和11.9%,并发现2例伯氏疏螺旋体和贝氏柯克斯体复合感染的情况。结论中蒙边境地区蜱携带伯氏疏螺旋体、贝氏柯克斯体和西伯利亚立克次体等病原体,应有针对性地加强当地蜱类和蜱传疾病的监测和防控。Objective To test for the presence of tick-borne pathogens in 162 adult ticks （Hyalomma asiaticum kozlovi ） collected at Ganqimaodu ports between Sino-Mongolia border in 2010. Methods RT-PCR was proceeded to detect tick borne encephalitis virus （TBEV）, Crimean-Congo haemorrhagic fever virus （CCHF）, Bunyaviruses; PCR was proceeded to detect Borrelia, Rickettsia sibirica, Coxiella burnetii, Anaplasma phagocytophilum from RNA and DNA derived from ticks respectively. Results RT-PCR and PCR results of TBEV, CCHF, Bunyaviruses and Anaplasma phagocytophilum were all negative , Borrelia was identified in 3.7% of ticks（5/162）, Rickettsia sibirica was identified in 2.2% of ticks（3/162）,Coxiella burnetii was identified in 11.9% of ticks（16/162）, Borrelia and Coxiella burnetii were found coexist in two ticks. Conclusion These findings show an active role of ticks in maintaining Borrelia, Rickettsia sibirica, Coxiella burnetii in the borders regions between Sino-Mongolia, It is necessary to strengthen tick-borne diseases surveillance in this region.

关 键 词：中蒙 口岸 亚东璃眼蜱 蜱传病原体 调查 

分 类 号：R184.39[医药卫生—流行病学]