高效液相色谱法测定复方二仙草胶囊中淫羊藿苷含量  

Determination of icariin in compound Erxiancao capsule by HPLC

作　　者：刘娜[1] 杨天鸣[1] 赵兴红[1] 盖静[1] 王俊丽[1] 

机构地区：[1]兰州军区联勤部药品仪器检验所,甘肃兰州730050

出　　处：《中兽医医药杂志》2011年第5期28-30,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：兰州军区医药科技项目

摘　　要：目的:建立HPLC测定复方二仙草胶囊中淫羊藿苷含量的方法。方法:Shim-pack VP-ODS C18分析色谱柱(4.6 mm×150 mm,5μm),Phenomenex ODS C18保护柱(3 mm×4 mm)。流动相:乙腈-水(26∶74),流速1.0 mL/min,检测波长270 nm。结果:淫羊藿苷保留时间为13.8 min。以峰面积Y对进样量X(μg)线性回归,淫羊藿苷回归方程:y=2 732.7x+12.860r,=1.000 0,线性范围0.1-1.5μg。淫羊藿苷回收率为102.16%,RSD为1.92%。结论:本方法操作简便,结果准确可靠,可用于复方二仙草胶囊中淫羊藿苷的含量测定。Aim： To develop a high-performance liquid chromatography（HPLC） method for the quantification of icariin in compound Erxiancao capsule.Methods： The chromatographic separation was performed on a Shim-pack VP-ODS C18 column（4.6 mm×150 mm,5 μm） and Phenomenex ODS C18 security guard column（3 mm×4 mm） using acetonitrile-water（26∶74） as mobile phase with a flow rate of 1.0 mL/min.The detection was carried out at 270 nm.Results： The retention time of icariin was 13.8 min.The regress equation for icariin was y=2 732.7 x＋12.860,r= 1.000 0.The assay exhibited a linear range from 0.1 to 1.5 μg.The average recovery of icariin was 102.16% with RSD of 1.92%.Conclusion： The assay proved to be accurate,sensitive,selective and convenient.The fully validated method can be applied to study the quality control of compound Erxiancao capsule.

关 键 词：HPLC 复文二仙草胶囊 淫羊藿苷 含量测定 

分 类 号：S853.74[农业科学—临床兽医学]