种植自体脂肪干细胞的可吸收性细胞支架移植行兔阴茎增粗术的安全有效性研究  

A Study of penis girth enhancement with PLGA biodegradable scaffold cografting with autologous ADSC

作　　者：崔万寿[1] 巩艳青[1] 金哲[2] 刘涛[1] 李维仁[1] 李广永[1] 周峰[1] 白光一[1] 刘京[1] 高喆珠[1] 辛钟成[1] 

机构地区：[1]北京大学第一医院男科中心,北京100009 [2]北京老年医院泌尿外科

出　　处：《中国男科学杂志》2011年第8期9-14,共6页Chinese Journal of Andrology

基　　金：基金项目资助：北京市自然科学基金（No.Z080507030808011）和国家自然科学基金（No.30972993）资助

摘　　要：目的观察自体脂肪干细胞（adipose derived stem cell，ADSC）种植可吸收性细胞支架对小阴茎增粗安全性和有效性。方法45只雄性成年新西兰白兔随机分为假手术对照组（A组）、单纯Maxpol-T移植组（B组）和种植自体ADSC的Maxpol-T移植组（C组）。静脉麻醉后取腹膜后脂肪组织，体外分离、鉴定和扩增ADSC并种植于可吸收性细胞支架Maxpol-T后，移植入兔阴茎皮下深筋膜和白膜之间。术后6个月评估阴茎增粗的效果及其安全性和有效性。结果ADSC培养后流式鉴定结果CD34（＋），CD90（＋），CD105（＋），CD133（＋），CD13（-），CD45（-）。移植术后6个月，三组均未发生支架排斥及炎症反应，A组无明显组织再生变化（P〉0．05），B组阴茎直径较A组无明显差异（P〉0．05），C组阴茎直径较A组和B组增粗效果显著（P〈0．001）。局部组织病理学结果可发现增生组织及新生血管。结论应用自体ADSC种植可吸收性细胞支架后植入成年新西兰白兔阴茎皮下，可安全有效地增生阴茎皮下局部组织，无明显排斥和炎症反应。Objective To evaluate the efficacy and safety of penis girth enhancement with PLGA Biodegradable Scaffold （Maxpol-T） cografting with adipose derived stem cells（ADSC）. Methods Forty-five adult New Zealand male rabbits were divided into three groups including the shame operation group （group A）, grafted Maxpol-T only group （group B） and Maxpol-T cografted with ADSC group （group C）. ADSC were isolated from the abdomen subcutaneous fat of rabbits and seeded into PLGA Scaffold, then it was transplanted into the deep fascia of penis. The efficacy and safety of girth enhancement were continuously observed for 6 months. Results The identification of the cultured ADSC by Flow Cytometry showed CD34（＋）, CD90（＋）, CD105（＋）, CD133（＋）, CD13（-） and CD45（-）. Six months later, no obvious rejection and inflammation were observed. The penis girth of group C was augmented significantly compared with that of group A and group B （P〈0.001）. There was no significant difference between the penis girth of group A and group B （P〉0.05）. Region histopathological analysis showed that subcutaneous tissue get thickened and desmoplasia accompanying with new vessels in group B and group C. Conclusion Penis girth enhancement with PLGA Biodegradable Scaffold cografting with ADSC on might be safe and effective.

关 键 词：干细胞 支架 阴茎 兔 

分 类 号：R697.1[医药卫生—泌尿科学]