益气化瘀补肾方对脊髓型颈椎病患者退变椎间盘细胞ColⅠ与ColⅡ mRNA表达的影响  

Effects of "Yiqi Huayu Bushen Recipe" on ColⅠ and ColⅡ mRNA Expressions of Degenerative Intervertebral Disc Cells in Patients with Cervical Spondylotic Myelopathy

作　　者：李具宝[1,2] 周泉[2] 赵永见[2] 赵红云[3] 李宁[1,2] 莫文[3] 张霆[3] 侯炜[3] 梁倩倩[2] 许鹏[4] 施杞[2] 王拥军[2] 

机构地区：[1]上海中医药大学附属龙华临床医学院,上海200032 [2]上海中医药大学脊柱病研究所,上海200032 [3]上海中医药大学附属龙华医院骨伤科,上海200032 [4]第二军医大学附属长征医院骨科,上海200003

出　　处：《上海中医药大学学报》2011年第5期61-65,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家教育部高等学校博士点基金资助项目(20070268004);上海市科委基础研究重点项目(07JC14050);国家重点基础研究计划("973"计划)基金资助项目(2010B530404);国家杰出青年科学基金资助项目(30625043);上海市高校创新团队计划项目(2009-6)

摘　　要：目的:观察益气化瘀补肾方含药血清对脊髓型颈椎病患者退变椎间盘细胞ColⅠ、ColⅡmRNA表达的影响。方法:选择脊髓型颈椎病患者经手术摘除的椎间盘组织,采用组织块法培养原代细胞;以SPF级雄性SD大鼠制备药物血清。将传代第1代的退变椎间盘细胞接种于96孔酶标板中,分为6组,分别予低(5%)、中(10%)、高(20%)浓度的益气化瘀补肾方含药血清及相应浓度生理盐水血清DMEM培养液培养。通过实时荧光定量PCR检测ColⅠ、ColⅡmRNA的表达。结果:不同浓度的益气化瘀补肾方血清对椎间盘细胞增殖有明显的促进作用;与生理盐水组比较,益气化瘀补肾方不同浓度含药血清干预后椎间盘细胞ColⅡmRNA表达水平上调、ColⅠmRNA表达水平下调,尤以中浓度更为明显(P<0.01)。结论:益气化瘀补肾方可能通过影响椎间盘组织胶原的变化而发挥防治脊髓型颈椎病椎间盘退变的作用。Objective：To investigate the effects of ＂Yiqi Huayu Bushen Recipe＂ containing serum on ColⅠ and ColⅡ mRNA expressions of the degenerative intervertebral disc cells in patients with cervical spondylotic myelopathy.Methods：The degenerative intervertebral disc tissues were obtained from the patients with cervical spondylotic myelopathy through surgery,and the primary cells were cultured by tissue mass method;the male SD rats of SPF level were chosen to prepare the drug serum.The degenerative intervertebral disc cells of the first generation were vaccinated in 96-well microtiter plates,divided into six groups,and cultured by low（5%）,medium（10%） and high（20%） concentration serum containing ＂Yiqi Huayu Bushen Recipe＂ and corresponding concentration serum containing normal saline,respectively.The Col Ⅰand Col Ⅱ mRNA expressions were detected by real-time quantitative PCR method.Results：The different concentration serum containing ＂Yiqi Huayu Bushen Recipe＂ could obviously promote proliferation of the intervertebral disc cells.Compared with the corresponding concentration serum containing normal saline groups,the Col Ⅱ mRNA expression was increased,and the Col Ⅰ mRNA expression was decreased in different concentration serum containing ＂Yiqi Huayu Bushen Recipe＂ groups,especially the medium concentration group was more obvious（P0.01）.Conclusion：＂Yiqi Huayu Bushen Recipe＂ may regulate collagen expression in degenerative intervertebral disc cells to prevent and treat cervical spondylotic myelopathy.

关 键 词：益气化瘀补肾方 脊髓型颈椎病 退变椎间盘细胞 ColⅠ ColⅡ 

分 类 号：R285.5[医药卫生—中药学] R274.9[医药卫生—中医学]