钙拮抗剂合心爽抑制动脉粥样硬化形成的实验研究  

The suppress effect of diltiazem on atherosclerosis in rabbits

作　　者：李永洪 宋道岭[2] 

机构地区：[1]菏泽鲁心心脑血管病医院心内科,山东省274016 [2]大连医科大学一附院心内科

出　　处：《中国心血管病研究》2011年第10期775-778,共4页Chinese Journal of Cardiovascular Research

摘　　要：目的 观察合心爽对家兔动脉粥样硬化（AS）形成的抑制作用.方法 28只家兔随机分为正常对照组（CTRL）、单纯胆固醇喂饲组（CHOL）、维生素D3组（CHVD）（胆固醇喂饲＋维生素D3 50 000 U/kg,im,每2周1次）及合心爽组（CHDL）（胆固醇喂饲＋合心爽100 mg·kg-1·d-1）4组,分别于第15、45、84天对比检测血清脂质过氧化物（LPO）、超氧化岐化酶（SOD）、过氧化氢酶（CAT）浓度变化,实验12周结束后对比观察各组间胸主动脉组织钙含量和主动脉组织AS病理损伤程度.结果 各组主动脉钙含量与AS损伤程度均呈正相关.与CHOL组指标进行比较,CHVD组主动脉钙含量、血LPO含量、AS病理损伤指数均较高（均P＜0.01）,而血SOD、CAT活性则较低（均P＜0.01）;CHDL组LPO含量、AS损伤指标明显低于CHOL组（均P＜0.01）,SOD、CAT活性反而高于CHOL组（均P＜0.01）.结论 AS病理的发生发展与动脉组织钙含量的增加有关.合心爽能够抑制AS的形成,减少动脉壁钙含量,并可提高血清SOD、CAT的浓度.Objective To evaluate the protective effect of diltiazem on atherosclerosis （AS） in rabbits. Methods 28 rabbits were randomly divided into four groups （n=7,in each）： CTRL group（control group）,CHOL group （only fed cholesterol）,CHVD group （besides fed cholesterol,vitamin D3 were given at 50 000 IU/kg, im, once 2 weeks） and CHDL group （fed cholesterol add diltiazem 100 mg/kg, once a day）. The parameters in serum including the levels of LPO, activity of SOD and CAT were detected respectively at 15 d, 45 d, 84 d of the experiment stage in all objects. After AS molding procedure, which was ended at the end of the 12th week, the histomorphology of AS lesion and the calcium content in aorta tissue were observed in all groups. Results The degree of AS lesion and calcium content of aorta wall in three treatment groups was higher than that in control group. Compared with these in CHOL group,the calcium content of arterial wall and AS indexes in CHVD group were very higher （all P〈0.05）, whereas these parameters in CHDL group showed significant lower （all P〈0.05）. Also there were significant different of the levels of LOP, SOD and CAT in serum between two groups whether CHOL and CHVD or CHOL and CHDL （all P〈0.05）. Conclusion This arterial calcium overload is accompanied by a progressive procedure of AS lesion. The use of diltiazem for treatment AS can suppressing AS procedure by decreasing the calcium content in aorta wall and increasing the serum levels of SOD and CAT.

关 键 词：钙超载 自由基 动脉粥样硬化 钙拮抗剂 维生素D3 

分 类 号：Q95-33[生物学—动物学] R541.4[医药卫生—心血管疾病]